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  1. Article

    Open Access

    Larval midgut modifications associated with Bti resistance in the yellow fever mosquito using proteomic and transcriptomic approaches

    Bacillus thuringiensis var. israelensis (Bti) is a natural larval mosquito pathogen producing pore-forming toxins targeting the midgut of Diptera larvae. It is used worldwide for mosquito control. Resistance mech...

    Guillaume Tetreau, Krishnareddy Bayyareddy, Christopher M Jones… in BMC Genomics (2012)

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    Article

    Comparison of the localization of Bacillus thuringiensis Cry1A δ-endotoxins and their binding proteins in larval midgut of tobacco hornworm, Manduca sexta

    Tobacco hornworm, Manduca sexta, is a model insect for studying the action of Bacillus thuringiensis (Bt) Cry toxins on lepidopterans. The proteins, which bind Bt toxins to midgut epithelial cells, are key factor...

    Jiang Chen, Mark R. Brown, Gang Hua, Michael J. Adang in Cell and Tissue Research (2005)

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    Chapter

    Investigations of Bacillus thuringiensis Cry1 toxin receptor structure and function

    Bacillus thuringiensis Cry1 proteins are highly toxic to some species of lepidopteran larvae. Cry proteins act on the brush border membrane through a multi-step process. The process includes Cry1 c...

    Stephen F. Garczynski, Michael J. Adang in Entomopathogenic Bacteria: from Laboratory… (2000)

  4. No Access

    Article

    Testing transgenes for insect resistance using Arabidopsis

    One possible strategy to delay the selection of resistant insect populations is the pyramiding of multiple resistance genes into a single cultivar. However, the transformation of most major crops remains prohi...

    Michael O. Santos, Michael J. Adang, John N. All, H. Roger Boerma… in Molecular Breeding (1997)

  5. No Access

    Article

    Expression of a Bacillus thuringiensis cryIA(c) gene in transgenic peanut plants and its efficacy against lesser cornstalk borer

    The invasion of peanut (Arachis hypogaea L.) pods and seeds by aflatoxin-forming species of Aspergillus is linked to injury by the lesser cornstalk borer and frequently causes a severe reduction in crop qualit...

    Chong Singsit, Michael J. Adang, Robert E. Lynch… in Transgenic Research (1997)

  6. No Access

    Article

    The reconstruction and expression of a Bacillus thuringiensis cryIIIA gene in protoplasts and potato plants

    A Bacillus thuringiensis (B.t.) cryIIIA δ-endotoxin gene was designed for optimal expression in plants. The modified cry gene has the codon usage pattern of an average dicot gene and does not contain AT-rich nucl...

    Michael J. Adang, Margaret S. Brody, Guy Cardineau, Nancy Eagan… in Plant Molecular Biology (1993)

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    Article

    Size and organization of a multigene family encoding phaseolin, the major seed storage protein of Phaseolus vulgaris L.

    Solution hybridization kinetics and genomic nitrocellulose blot hybridization analyses show that the Phaseolus vulgaris L. (French bean) storage proteins (phaseolins) are encoded as a small, homologous, multigene...

    Duncan R. Talbot, Michael J. Adang, Jerry L. Slightom… in Molecular and General Genetics MGG (1984)

  8. No Access

    Chapter

    An Insect Virus for Genetic Engineering: Develo** Baculovirus Polyhedrin Substitution Vectors

    Baculoviruses are exceptionally attractive candidates as vectors for propagating and expressing exogenous DNAs in a eukaryotic (invertebrate) environment (Miller, 1981a). Among the features which make baculovi...

    Lois K. Miller, David W. Miller, Michael J. Adang in Genetic Engineering in Eukaryotes (1983)