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Article
Characterization of Insertion Sites in Rainbow Papaya, the First Commercialized Transgenic Fruit Crop
Inserts and insert sites in transgenic, papaya ringspot virus (PRSV)-resistant commercial papaya Rainbow and SunUp, were characterized as part of a petition to Japan to allow import of fresh fruit of these cul...
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Article
Pathogen-derived resistance provides papaya with effective protection against papaya ringspot virus
Transgenic Carica papaya plants (cv. Sunset, R0 clone 55-1) carrying the coat protein gene of papaya ringspot virus (strain HA 5-1) remained symptomless and ELISA-negative for 24 months after inoculation with ...
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Article
Biolistic transformation of chrysanthemum with the nucleocapsid gene of tomato spotted wilt virus
In vitro regeneration and biolistic transformation procedures were developed for several commercial chrysanthemum Dendranthema grandiflora Tzvelev, syn. Chrysanthemum morifolium Ramat. cultivars using leaf and s...
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Protocol
Transformation of Soybean (Glycine max) via Agrobacterium tumefaciens and Analysis of Transformed Plants
The transfer of genetic material into soybean (Glycine max L. Merr.) plant tissues has been accomplished by several methods: electroporation (1), microprojectiles (2), and by the more widely used Agrobacterium-me...
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Article
Different Mechanisms Protect Transgenic Tobacco Against Tomato Spotted Wilt and Impatiens Necrotic Spot Tospoviruses
We generated transgenic tobacco plants expressing the sense or antisense untranslatable N coding sequence of the lettuce isolate of tomato spotted wilt virus (TSWV-BL) as well as transgenic plants containing t...
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Article
Transgenic papaya plants from Agrobacterium-mediated transformation of somatic embryos
Transgenic papaya (Carica papaya L.) plants were regenerated from embryogenic cultures that were cocultivated with a disarmed C58 strain of Agrobacterium tumefaciens containing one of the following binary cosmid ...
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Article
Comparison of Coat Protein-Mediated and Genetically-Derived Resistance in Cucumbers to Infection by Cucumber Mosaic Virus Under Field Conditions with Natural Challenge Inoculations by Vectors
During three consecutive growing seasons, transgenic cucumbers of the cultivar Poinsett 76 possessing the coat protein gene of a non-aphid transmissible strain of cucumber mosaic virus (CMV) were compared with...
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Article
Virus Resistant Papaya Plants Derived from Tissues Bombarded with the Coat Protein Gene of Papaya Ringspot Virus
Papaya ringspot virus (PRV) is a serious disease of papaya (Carica papaya L.) that has only been partially controlled by conventional methods. An alternative control method is coat protein-mediated protection (C...
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Article
Sequence analysis of linked maize 22 kDa α-zein genes
We have determined the nucleotide sequence of a 7343 bp zein genomic clone (gZ22.8H3) from the maize inbred W64A. Computer-aided analysis of the DNA sequence revealed two contiguous 22 kDa α-zein genes. The 5′...
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Chapter
Field testing transgenic cucumber plants that express the CMV coat protein gene
The transfer and expression of the gene that encodes the coat protein of plant viruses in plants has been very successful for the development of plants that are protected against subsequent infections by the s...
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Article
Protection Against Detrimental Effects of Potyvirus Infection in Transgenic Tobacco Plants Expressing the Papaya Ringspot Virus Coat Protein Gene
We obtained transgenic tobacco plants expressing the papaya ringspot virus (PRV) coat protein (CP) gene by transformation via Agrobacterium tumefaciens. Expression was effectively monitored by enzyme-linked immun...
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Chapter
Custom polymerase chain reaction engineering plant expression vectors and genes for plant expression
In Chapter C3 we have shown how the polymerase chain reaction (PCR) amplifi-cation can be an extremely useful technique for the identification of transgenic plants. In this chapter we continue to demonstrate t...
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Chapter
Molecular Biology of Legume Vicilin-Type Seed Storage Protein Genes
The major seed storage proteins of legumes are globulins (packaged into cotyledonary protein storage bodies), which are represented in most legumes by two different types of polypeptides; the nonglycosylated 1...
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Chapter
Using polymerase chain reaction to identify transgenic plants
The ability to transfer genes into many plant species is well established. The more commonly used techniques include Agrobacterium tumefaciens T-DNA-mediated transfer [3, 15, 16], direct DNA transfer into protopl...
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Article
Stable transformation of papaya via microprojectile bombardment
Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly expl...
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Article
Primate evolution at the DNA level and a classification of hominoids
The genetic distances among primate lineages estimated from orthologous noncoding nucleotide sequences of β-type globin loci and their flanking and intergenic DNA agree closely with the distances (delta T50H valu...
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Chapter
Field Testing of Cucumber Plants Which Express the CMV Coat Protein Gene: Field Plot Design to Test Natural Infection Pressures
The development of plants which are tolerant to virus infections has been shown by the expression of the tobacco mosaic virus (TMV) coat protein gene in transgenic tobacco plants (1). However, TMV is not the m...
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Article
Analysis of TR-DNA/plant junctions in the genome of a Convolvulus arvensis clone transformed by Agrobacterium rhizogenes strain A4
A Charon 4A phage library, containing insert DNA isolated from a morning glory (Convolvulus arvensis) plant genetically transformed by Ri T-DNA from Agrobacterium rhizogenes strain A4, was used to isolate a lambd...
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Chapter
Procedures for constructing genomic clone banks
The construction of recombinant genomic DNA clone banks containing complete plant genomes followed the construction of similar clone banks with mammalian DNAs by about three years. The reason for this lag peri...
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Chapter
Procedures for constructing ds-cDNA clone banks
The construction of the first plant ds-cDNA clones used the abundant mRNAs which encode bean seed storage proteins of Phaseolus vulgaris and Glycine max as starting materials [11, 32, 33]. These mRNAs comprise ab...
