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Article
BARD1 reads H2A lysine 15 ubiquitination to direct homologous recombination
Protein ubiquitination at sites of DNA double-strand breaks (DSBs) by RNF168 recruits BRCA1 and 53BP11,2, which are mediators of the homologous recombination and non-homologous end joining DSB repair pathways, re...
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Article
Open AccessPALB2 chromatin recruitment restores homologous recombination in BRCA1-deficient cells depleted of 53BP1
Loss of functional BRCA1 protein leads to defects in DNA double-strand break (DSB) repair by homologous recombination (HR) and renders cells hypersensitive to poly (ADP-ribose) polymerase (PARP) inhibitors use...
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Article
Open AccessMDC1 PST-repeat region promotes histone H2AX-independent chromatin association and DNA damage tolerance
Histone H2AX and MDC1 are key DNA repair and DNA-damage signalling proteins. When DNA double-strand breaks (DSBs) occur, H2AX is phosphorylated and then recruits MDC1, which in turn serves as a docking platfor...
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Article
Open AccessRetroviral integration into nucleosomes through DNA loo** and sliding along the histone octamer
Retroviral integrase can efficiently utilise nucleosomes for insertion of the reverse-transcribed viral DNA. In face of the structural constraints imposed by the nucleosomal structure, integrase gains access t...
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Article
Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR–Cas9 genome-editing efficiency
Modulation of DNA repair pathway choice by a potent inhibitor of 53BP1 improves the efficiency of homology-dependent genome editing in human and mouse cells.
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Article
The structural basis of modified nucleosome recognition by 53BP1
A cryo-electron microscopy structure of the DNA damage repair protein 53BP1 bound to a nucleosome illuminates the way 53BP1 recognizes two types of histone modifications (a methyl group and a ubiquitin moiety)...
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Article
A mechanism for the suppression of homologous recombination in G1 cells
A mechanism for the repression of homologous recombination in G1, the stage of the cell cycle preceding replication, is determined; the critical aspects are the interaction between BRCA1 and PALB2–BRCA2, and s...
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Article
A Pharmacoepidemiological Network Model for Drug Safety Surveillance
Background: Recent withdrawals of major drugs have highlighted the critical importance of drug safety surveillance in the postmarketing phase. Limitations of spontaneous report data have led drug...
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Article
Building the Outcomes-Based Formulary
The traditional formulary review process possesses limitations that impair a Pharmacy and Therapeutics committee’s ability to thoroughly evaluate products, including the lack of emphasis on a product’s impact ...
