34 Result(s)
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M. A. Khodorkovskii
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Article
Type II CRISPR–Cas System Nucleases: A Pipeline for Prediction and In Vitro Characterization
The use of CRISPR–Cas bacterial adaptive immunity system components for targeted DNA changes has opened broad prospects for programmable genome editing of higher organisms. The most widely used gene editors ar...
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Article
Mechanisms of Bacterial Cell Division
Despite numerous studies, gaps still remain in our understanding of bacterial cell division. This review describes the basic mechanisms responsible for division of the bacterial cell and coordination of this p...
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Influence of FtsZ proteins from some mycoplasma species on the division process in Escherichia coli cells
FtsZ is a highly conserved protein that performs one of the key roles in cell division of most prokaryotes. At the present time it is believed that the main role of FtsZ in the cells of the microorganisms stud...
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Article
A truncated form of α-tubulin detected in purified proteasome complexes
The 26S proteasome is a multisubunit protein complex responsible for selective protein degradation in the cell. A number of proteins with known and unknown functions were shown to be permanently or temporarily...
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Recovery of division process in bacterial cells after induction of SulA protein which is responsible for cytokinesis arrest during SOS-response
SOS-response is an important tool of bacteria intended to protect their genome and thereby allow them to survive under adverse conditions. Recently SOS-response was demonstrated to enhance mutagenesis and thus...
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New data on structures formed by the FtsZ protein during the cell division process in Escherichia coli obtained using the localization microscopy method
The FtsZ protein, a bacterial tubulin homolog, is one of the key proteins in bacterial cell division, forming a contractile Z-ring in the middle of the dividing cell. In the present study, immunofluorescence s...
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Article
Chemical structure and physicochemical properties of oxidized hydrolysis lignin
The structure of hydrolysis lignin oxidized with hydrogen peroxide in acid medium was studied by NMR spectroscopy and by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometr...
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Article
Discharge plasma in an argon–water vapor mixture as a gas laser medium
The possibility of obtaining the inverse population of the excited states of hydroxyl molecules generated in low-pressure discharge plasma in an Ar–H2O mixture was studied. The possibility of inversion of both th...
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Article
Mass spectrometric analysis of affinity-purified proteasomes from the human myelogenous leukemia K562 cell line
Proteasomes carry out regulated proteolysis of most proteins in a cell and thereby play a crucial role in the regulation of various cellular processes. Determination of the subunit composition and posttranslat...
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Article
Structural and functional characteristics of various forms of red pigment of yeast Saccharomyces cerevisiae and its synthetic analog
Structural and functional characteristics of the yeast red pigment (product of polymerization of N1-(β-D-ribofuranosyl)-5-aminoimidazole), isolated from ade1 mutant cells of Saccharomyces cerevisiae and its derib...
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Article
Investigation of carbon graphite-like structures by laser mass spectrometry
Sorbents prepared from industrial waste and some carbon graphite-like materials are studied by laser ablation and time-of-flight mass spectrometry. The mass spectra of all materials show a peak at a mass of 46...
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Article
The study of ungerade electronic states of the Xe2 molecules in the region of Xe*(5p 56p, 5d, 7s, 6d) by the resonance multiphoton ionization method
Electronic spectra of the Xe2 molecules in the energy range of 77700–89300 cm−1 are recorded. The method of resonance enhanced multiphoton ionization of molecules in a supersonic molecular beam was used, in which...
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Article
Effect of red pigment on insulin fibril formation in vitro
The influence of red pigment isolated from yeast Saccharomyces cerevisiae and its low molecular weight derivate on insulin amyloid fibril formation in vitro was studied. The red pigment derivative, which presumab...
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Article
Electronic spectra of XeNe molecules in the range 77100-90100 cm-1 near Xe* (6p, 5d, 6p’, 7s, 7p, 6d) obtained by the (3 + 1) REMPI and (2 + 1) REMPI methods
The electronic spectra of XeNe molecules in the range of 77100-90100 cm-1 are measured by the method of laser resonance multiphoton ionization in a supersonic jet. The photoionization spectra are obtained upon tw...
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Article
Laser mass spectrometry study of the properties of fullerene structures
Fullerene films grown by various methods are studied using mass spectrometry. The mass spectra of the films formed onto an aluminum foil using thermal deposition (TD) or supersonic molecular beam (SMB) exhibit...
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Article
Electron impact ionization of Ar n , (H2O) n , Ar n (H2O) m clusters
Signals from ions forming in a supersonic molecular beam consisting of an argon-water vapor mixture are measured as functions of the exciting electron energy in the range to 120 eV. The thresholds of electron ...
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Article
Study of the lowest electronic states of Xe2, XeKr, and XeAr molecules by the method of multiphoton resonance ionization
The spectra of Xe2, XeKr, and XeAr molecules in the range 66 500–68 800 cm−1 are obtained by the methods of (2 + n) and (3 + n) (n = 1, 2, 3) resonance multiphoton ionization during the registration of molecular ...
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Article
Composition of a water vapor-argon mixture determined by the mass spectrometry of a supersonic molecular beam
It is found that the composition of a pulsed supersonic molecular beam depends on the preparation conditions of the initial mixture containing water molecules and argon atoms. The mass spectra of the molecular...
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Article
Multiphoton mass spectra of XeKr molecules in the range of excited Xe*6p[5/2]2, 3 atoms
Data on excited states of XeKr molecules in the energy range 78280–77600 cm−1 are obtained. Using the method of multiphoton laser photoionization of molecules in a supersonic jet, five vibrational progressions of...
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Article
Properties of fullerene films obtained by high-kinetic-energy deposition on various surfaces
Fullerene films deposited at various kinetic energies are studied by X-ray photoelectron spectroscopy. The structure of the first monolayer of fullerene molecules is shown to control the growth and structure o...
