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Article
A truncated form of α-tubulin detected in purified proteasome complexes
The 26S proteasome is a multisubunit protein complex responsible for selective protein degradation in the cell. A number of proteins with known and unknown functions were shown to be permanently or temporarily...
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Article
Recombinant small heat shock protein from Acholeplasma laidlawii increases the Escherichia coli viability in thermal stress by selective protein rescue
In both prokaryotes and eukaryotes, the survival at temperatures considerably exceeding the optimum is supported by intense synthesis of the so-called heat shock proteins (HSPs), which act to overcome the adve...
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Article
New data on structures formed by the FtsZ protein during the cell division process in Escherichia coli obtained using the localization microscopy method
The FtsZ protein, a bacterial tubulin homolog, is one of the key proteins in bacterial cell division, forming a contractile Z-ring in the middle of the dividing cell. In the present study, immunofluorescence s...
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Article
Chemical structure and physicochemical properties of oxidized hydrolysis lignin
The structure of hydrolysis lignin oxidized with hydrogen peroxide in acid medium was studied by NMR spectroscopy and by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometr...
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Article
Mass spectrometric analysis of affinity-purified proteasomes from the human myelogenous leukemia K562 cell line
Proteasomes carry out regulated proteolysis of most proteins in a cell and thereby play a crucial role in the regulation of various cellular processes. Determination of the subunit composition and posttranslat...
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Article
Biosynthesis of hexa- and pentameric chitooligosaccharides using N-acetyl-glucoseaminyl transferase from rhizobial bacteria
Chitooligosaccharides are widely used, which provides considerable interest in their biosynthesis. Enzymatic synthesis of hexa-N-acetylchitohexaose and penta-N-acetylchitopentaose using N-acetylglucoseaminyl t...
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Article
Structural and functional characteristics of various forms of red pigment of yeast Saccharomyces cerevisiae and its synthetic analog
Structural and functional characteristics of the yeast red pigment (product of polymerization of N1-(β-D-ribofuranosyl)-5-aminoimidazole), isolated from ade1 mutant cells of Saccharomyces cerevisiae and its derib...
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Article
Purification and in vitro analysis of exosomes secreted by malignantly transformed human cells
Exosomes are natural nanoparticles secreted by different cells and capable of carrying protein markers and genetic information, thus participating in cellular communication. There is good reason to think that ...
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Article
Investigation of carbon graphite-like structures by laser mass spectrometry
Sorbents prepared from industrial waste and some carbon graphite-like materials are studied by laser ablation and time-of-flight mass spectrometry. The mass spectra of all materials show a peak at a mass of 46...
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Article
Effect of red pigment on insulin fibril formation in vitro
The influence of red pigment isolated from yeast Saccharomyces cerevisiae and its low molecular weight derivate on insulin amyloid fibril formation in vitro was studied. The red pigment derivative, which presumab...
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Article
Electronic spectra of XeNe molecules in the range 77100-90100 cm-1 near Xe* (6p, 5d, 6p’, 7s, 7p, 6d) obtained by the (3 + 1) REMPI and (2 + 1) REMPI methods
The electronic spectra of XeNe molecules in the range of 77100-90100 cm-1 are measured by the method of laser resonance multiphoton ionization in a supersonic jet. The photoionization spectra are obtained upon tw...
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Article
Laser mass spectrometry study of the properties of fullerene structures
Fullerene films grown by various methods are studied using mass spectrometry. The mass spectra of the films formed onto an aluminum foil using thermal deposition (TD) or supersonic molecular beam (SMB) exhibit...
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Article
Electron impact ionization of Ar n , (H2O) n , Ar n (H2O) m clusters
Signals from ions forming in a supersonic molecular beam consisting of an argon-water vapor mixture are measured as functions of the exciting electron energy in the range to 120 eV. The thresholds of electron ...
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Article
Composition of a water vapor-argon mixture determined by the mass spectrometry of a supersonic molecular beam
It is found that the composition of a pulsed supersonic molecular beam depends on the preparation conditions of the initial mixture containing water molecules and argon atoms. The mass spectra of the molecular...
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Article
Properties of fullerene films obtained by high-kinetic-energy deposition on various surfaces
Fullerene films deposited at various kinetic energies are studied by X-ray photoelectron spectroscopy. The structure of the first monolayer of fullerene molecules is shown to control the growth and structure o...
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Article
Multiphoton mass spectra of Xe2 molecules in the range of excited Xe*(6p, 5d) atoms
The (2 + 1) photoionization mass spectra of Xe2 molecules are studied in a supersonic jet upon excitation by laser radiation in the energy range 80321.3–77821 cm−1, corresponding to the dissociation of the Xe2 mo...
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Article
Composition of higher fullerenes obtained by laser ablation of carboniferous materials
The compositions of higher fullerenes produced by ablation of various carboniferous materials are experimentally studied. The yield of fullerenes versus the type of carboniferous materials and laser radiation ...
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Article
Dendrimers Based on α-Amino Acids: Synthesis and Hydrodynamic Characteristics
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Article
The binding energy of molecules in thin fullerene films
Thin fullerene films formed using the methods of pulsed supersonic molecular beam (SMB) deposition and thermal deposition (TD) in vacuum were studied by thermodesorption (TDS) mass spectroscopy. The TDS spectr...
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Article
Fullerene films highly resistant to laser radiation
The effect of laser radiation on the structure and phase composition of thin fullerene films is studied. Raman measurements show that fullerene films applied with a supersonic molecular beam remain structurall...