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Article
Open AccessProtein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview
Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or indu...
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Article
Open AccessMacromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels
Analysis of the cell operation at the metabolic level requires collecting data of different types and to determine their confidence level. In addition, the acquired information has to be combined in order to o...
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Article
Nulliparous patients with small stature delivering at term have an increased risk of secondary cesarean section
To evaluate the obstetric outcome of pregnant patients with small stature (<5th percentile) with regard to the mode of delivery, maternal injuries, and neonatal parameters.
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Article
Open AccessA multi-level study of recombinant Pichia pastoris in different oxygen conditions
Yeasts are attractive expression platforms for many recombinant proteins, and there is evidence for an important interrelation between the protein secretion machinery and environmental stresses. While adaptive...
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Article
Open AccessThe impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis
Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for the production of recombinant proteins. Their known genome sequences enabled several transcriptomic pro...
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Article
Coexistent parathyroid adenoma and thyroid papillary carcinoma in pregnancy
Primary hyperparathyroidism in pregnant women is a rare antenatal complication. Maternal symptoms include nephrolithiasis, bone disease, pancreatitis, hyperemesis, muscle weakness, mental status changes, and h...
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Article
Open AccessProtein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris
The increasing availability of 'omics' databases provide important platforms for yeast engineering strategies since they offer a lot of information on the physiology of the cells under diverse growth condition...
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Article
Reproducibility of fetal ductus venosus blood flow velocity waveforms during first stage of labor
Fetal ductus venosus (DV) blood flow velocity waveforms are significantly altered during contractions in first stage of labor. We have evaluated the reproducibility of these waveforms during and between contra...
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Chapter and Conference Paper
Distribution of Superparamagnetic Nanoparticles in Lymphatic Tissue for Sentinel Lymph Node Detection in Breast Cancer by Magnetic Particle Imaging
Breast cancer diagnostic and treatment consists of surgical tumor removal and axillary lymph node resection. Radical axillary lymph node removemend is associated with high morbidity and significant loss of qua...
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Article
The prognostic impact of age in different molecular subtypes of breast cancer
Breast cancer is a heterogeneous entity composed of distinct molecular subgroups with different molecular and clinical features. We analyzed the association between molecular breast cancer subgroups, age at di...
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Chapter
Congenital and Developmental Abnormalities of the Breast
Congenital abnormities of the breast rarely occur. They can be split into three main groups: (1) excessive development within the field of the embryonic milk line, containing polythelia (development of an addi...
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Article
Cosmetic changes following surgery and accelerated partial breast irradiation using HDR interstitial brachytherapy
Patients with early-stage breast cancer can benefit from adjuvant accelerated partial breast irradiation (APBI) following breast-conserving surgery (BCS). This work reports on cosmetic results following APBI u...
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Article
Open AccessGoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris
State-of-the-art strain engineering techniques for the host Pichia pastoris (syn. Komagataella spp.) include overexpression of homologous and heterologous genes, and deletion of host genes. For metabolic and cell...
