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Article
Open AccessThe adaptive landscape of wildtype and glycosylation-deficient populations of the industrial yeast Pichia pastoris
The effects of long-term environmental adaptation and the implications of major cellular malfunctions are still poorly understood for non-model but biotechnologically relevant species. In this study we perform...
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Article
Open AccessImplications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris
Pichia pastoris is a widely used eukaryotic expression host for recombinant protein production. Adaptive laboratory evolution (ALE) has been applied in a wide range of studies in order...
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Article
Open AccessRecombinant Aspergillus β-galactosidases as a robust glycomic and biotechnological tool
Galactosidases are widespread enzymes that are used for manifold applications, including production of prebiotics, biosynthesis of different transgalactosylated products, improving lactose tolerance and in var...
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Article
Open AccessAdaptive laboratory evolution – principles and applications for biotechnology
Adaptive laboratory evolution is a frequent method in biological studies to gain insights into the basic mechanisms of molecular evolution and adaptive changes that accumulate in microbial populations during l...
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Article
Open AccessA synthetic biology approach to self-regulatory recombinant protein production in Escherichia coli
Recombinant protein production is a process of great industrial interest, with products that range from pharmaceuticals to biofuels. Since high level production of recombinant protein imposes significant stres...
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Article
Open AccessThe impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis
Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for the production of recombinant proteins. Their known genome sequences enabled several transcriptomic pro...
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Article
Open AccessA multi-level study of recombinant Pichia pastoris in different oxygen conditions
Yeasts are attractive expression platforms for many recombinant proteins, and there is evidence for an important interrelation between the protein secretion machinery and environmental stresses. While adaptive...
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Article
Open AccessThe response to unfolded protein is involved in osmotolerance of Pichia pastoris
The effect of osmolarity on cellular physiology has been subject of investigation in many different species. High osmolarity is of importance for biotechnological production processes, where high cell densitie...
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Article
Open AccessGenome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris
Pichia pastoris is widely used as a production platform for heterologous proteins and model organism for organelle proliferation. Without a published genome sequence available, strain and process development reli...
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Article
Open AccessNovel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays
DNA Microarrays are regarded as a valuable tool for basic and applied research in microbiology. However, for many industrially important microorganisms the lack of commercially available microarrays still hamp...
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Article
Open AccessProtein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview
Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or indu...
