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Article

GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

State-of-the-art strain engineering techniques for the host Pichia pastoris (syn. Komagataella spp.) include overexpression of homologous and heterologous genes, and deletion of host genes. For metabolic and cell...

Roland Prielhofer, Juan J. Barrero, Stefanie Steuer, Thomas Gassler… in BMC Systems Biology (2017)