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Article
Chloroplast-actin filaments decide the direction of chloroplast avoidance movement under strong light in Arabidopsis thaliana
Chloroplast-actin (cp-actin) filaments are crucial for light-induced chloroplast movement, and appear in the front region of moving chloroplasts when visualized using GFP-mouse Talin. They are short and thick,...
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Article
Apodized phase contrast microscopy for bright high contrast reveals motion of organelles
We have developed a high magnification objective lens performed with apodized phase contrast microscopy for bright high contrast, aiming to image finer intact intracellular structures and to analyze organelles...
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Article
Open AccessRNaseH2A downregulation drives inflammatory gene expression via genomic DNA fragmentation in senescent and cancer cells
Cellular senescence caused by oncogenic stimuli is associated with the development of various age-related pathologies through the senescence-associated secretory phenotype (SASP). SASP is mediated by the activ...
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Article
Open AccessInternal constraints and arrested relaxation in main-chain nematic elastomers
Nematic liquid crystal elastomers (N-LCE) exhibit intriguing mechanical properties, such as reversible actuation and soft elasticity, which manifests as a wide plateau of low nearly-constant stress upon stretc...
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Article
Open AccessCoefficient of variation as an image-intensity metric for cytoskeleton bundling
The evaluation of cytoskeletal bundling is a fundamental experimental method in the field of cell biology. Although the skewness of the pixel intensity distribution derived from fluorescently-labeled cytoskele...
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Article
Open AccessWipi3 is essential for alternative autophagy and its loss causes neurodegeneration
Alternative autophagy is an Atg5/Atg7-independent type of autophagy that contributes to various physiological events. We here identify Wipi3 as a molecule essential for alternative autophagy, but which plays m...
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Protocol
Conventional, Apodized, and Relief Phase-Contrast Microscopy
Non-absorbing colorless specimens (phase objects) can be visualized either by chemical staining (histology) or by the so-called optical contrasting (staining). The latter is achieved by converting optical phas...
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Article
Open AccessArabidopsis vegetative actin isoforms, AtACT2 and AtACT7, generate distinct filament arrays in living plant cells
Flowering plants express multiple actin isoforms. Previous studies suggest that individual actin isoforms have specific functions; however, the subcellular localization of actin isoforms in plant cells remains...
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Article
Open AccessRibosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency
Recently, we reported that bacterial incorporation induces cellular transdifferentiation of human fibroblasts. However, the bacterium-intrinsic cellular- transdifferentiation factor remained unknown. Here, we ...
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Article
Open AccessUncovering different states of topological defects in schlieren textures of a nematic liquid crystal
Topological defects are ubiquitously found in physical systems and therefore have been an important research subject of not only condensed matter physics but also cosmology. However, their fine structures rema...
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Article
Open AccessFluorescence microscopy reveals molecular localisation at line defects in nematic liquid crystals
Topological defects easily form in liquid crystals (LCs) as a result of frustrations in spatially dependent anisotropic molecular ordering, and have been regarded as promising tools for facilitating manipulati...
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Article
Carbon fiber technique for the investigation of single-cell mechanics in intact cardiac myocytes
This protocol describes a method for attaching single isolated cardiac myocytes to carbon fibers for mechanical manipulation and measurement. This method relies on cell-adhesive carbon fibers that attach easil...
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Chapter and Conference Paper
Actin Dynamics in Neuronal Growth Cone Revealed With a Polarized Light Microscopy
More than 100 years ago, S.R. y Cajal1 discovered an enlargement of cytoplasm at the tip of neunte through a careful observation of the fixed nervous tissues. He named this structure as growth cone. He suggested ...
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Article
The Annual Meeting on Muscle Contractionand Cell Motility, TOKYO, JAPAN, 6–8 JANUARY 2001
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Article
Effect of caffeine on cytosolic Ca2+ level and cytoplasmic streaming inNitella axilliformis
The effect of caffeine on cytosolic Ca2+ level and rate of cytoplasmic streaming was studied inNitella. External application of caffeine induced both a transient rise in cytosolic Ca2+ level and a decline in the ...
