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Open AccessComprehensive and quantitative analysis of intracellular structure polarization at the apical–basal axis in elongating Arabidopsis zygotes
A comprehensive and quantitative evaluation of multiple intracellular structures or proteins is a promising approach to provide a deeper understanding of and new insights into cellular polarity. In this study,...
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Article
Machine learning and feature analysis of the cortical microtubule organization of Arabidopsis cotyledon pavement cells
The measurement of cytoskeletal features can provide valuable insights into cell biology. In recent years, digital image analysis of cytoskeletal features has become an important research tool for quantitative...
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Article
Open AccessAb-GALFA, A bioassay for insect gall formation using the model plant Arabidopsis thaliana
Insect galls are abnormal plant organs formed by gall-inducing insects to provide shelter and nutrients for themselves. Although insect galls are spatialized complex structures with unique shapes and function...
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Article
Open AccessDisease-associated H58Y mutation affects the nuclear dynamics of human DNA topoisomerase IIβ
DNA topoisomerase II (TOP2) is an enzyme that resolves DNA topological problems and plays critical roles in various nuclear processes. Recently, a heterozygous H58Y substitution in the ATPase domain of human T...
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Article
Open AccessBone marrow imaging reveals the migration dynamics of neonatal hematopoietic stem cells
Hematopoietic stem cells (HSCs) are produced from the blood vessel walls and circulate in the blood during the perinatal period. However, the migration dynamics of how HSCs enter the bone marrow remain elusive...
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Article
Two-step regulation of centromere distribution by condensin II and the nuclear envelope proteins
The arrangement of centromeres within the nucleus differs among species and cell types. However, neither the mechanisms determining centromere distribution nor its biological significance are currently well un...
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Protocol
Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic Tobacco BY-2 Cells
Transgenic tobacco BY-2 cell lines stably expressing fluorescent protein-tagged marker proteins have been used to visualize the dynamic behaviors of cytoskeletons and organelles during plant cell division. Usi...
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Article
Open AccessDiscovery, characterization and functional improvement of kumamonamide as a novel plant growth inhibitor that disturbs plant microtubules
The discovery and useful application of natural products can help improve human life. Chemicals that inhibit plant growth are broadly utilized as herbicides to control weeds. As various types of herbicides are...
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Article
Open AccessCoefficient of variation as an image-intensity metric for cytoskeleton bundling
The evaluation of cytoskeletal bundling is a fundamental experimental method in the field of cell biology. Although the skewness of the pixel intensity distribution derived from fluorescently-labeled cytoskele...
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Article
Open AccessArabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity
Pattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-trig...
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Article
Open AccessCorrection to: Theoretical models for branch formation in plants
The article Theoretical models for branch formation in plants
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Article
Open AccessTheoretical models for branch formation in plants
Various branch architectures are observed in living organisms including plants. Branch formation has traditionally been an area of interest in the field of developmental biology, and theoretical approaches are...
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Article
Author Correction: PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis
Root hairs elongate by tip growth and simultaneously harden the shank by constructing the inner secondary cell wall layer. While much is known about the process of tip growth1, almost nothing is known about the m...
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Article
Cell wall accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated Arabidopsis leaf epidermal cells
In most dicotyledonous plants, leaf epidermal pavement cells develop jigsaw puzzle-like shapes during cell expansion. The rapid growth and complicated cell shape of pavement cells is suggested to be achieved b...
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Article
Quantitative analyses on dynamic changes in the organization of host Arabidopsis thaliana actin microfilaments surrounding the infection organ of the powdery mildew fungus Golovinomyces orontii
Obligate biotrophic fungi that cause powdery mildew on host plants form a specialized infection organ called the haustorium in the host apoplast. It was previously reported that the haustorium is surrounded by...
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Article
Open AccessSemi-automatic organelle detection on transmission electron microscopic images
Recent advances in the acquisition of large-scale datasets of transmission electron microscope images have allowed researchers to determine the number and the distribution of subcellular ultrastructures at bot...
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Chapter
Programmed Cell Death in Plant Immunity: Cellular Reorganization, Signaling, and Cell Cycle Dependence in Cultured Cells as a Model System
Plants have evolved various means for controlled and organized cell destruction, known as programmed cell death (PCD). PCD is a crucial event in plant immunity against microbial infection that prevents the spr...
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Article
Open AccessA Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses
Plants control CO2 uptake and water loss by modulating the aperture of stomata located in the epidermis. Stomatal opening is initiated by the activation of H+-ATPases in the guard-cell plasma membrane. In contras...
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Article
Open AccessLIPS database with LIPService: a microscopic image database of intracellular structures in Arabidopsis guard cells
Intracellular configuration is an important feature of cell status. Recent advances in microscopic imaging techniques allow us to easily obtain a large number of microscopic images of intracellular structures....
