44 Result(s)
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Juan Carlos Izpisúa Belmonte
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Article
Open Accessc-MYC Triggers Lipid Remodelling During Early Somatic Cell Reprogramming to Pluripotency
Metabolic rewiring and mitochondrial dynamics remodelling are hallmarks of cell reprogramming, but the roles of the reprogramming factors in these changes are not fully understood. Here we show that c-MYC indu...
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Article
Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells
The generation of organoids is one of the biggest scientific advances in regenerative medicine. Here, by lengthening the time that human pluripotent stem cells (hPSCs) were exposed to a three-dimensional micro...
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Article
Erratum to: Generation of Pig iPS Cells: A Model for Cell Therapy
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Article
Open AccessSkeletal muscle regeneration in Xenopus tadpoles and zebrafish larvae
Mammals are not able to restore lost appendages, while many amphibians are. One important question about epimorphic regeneration is related to the origin of the new tissues and whether they come from mature ce...
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Chapter
Plucked hair: how to get stem cells and induced pluripotent stem cells for future clinical applications
Generation of induced pluripotent stem cells from somatic cells by the expression of defined transcription factors has created new opportunities for the development of patient-specific therapies. Thus far, the...
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Article
Diseases in a dish: modeling human genetic disorders using induced pluripotent cells
This review provides a guide to the conceptual and practical issues to consider when trying to generate an iPSc model that accurately recapitulates the features of a human genetic disease. The authors highligh...
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Article
Generation of Pig iPS Cells: A Model for Cell Therapy
Reprogramming of pig somatic cells to induced pluripotent stem cells provides a tremendous advance in the field of regenerative medicine since the pig represents an ideal large animal model for the preclinical...
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Article
Methods for making induced pluripotent stem cells: reprogramming à la carte
Direct reprogramming enables the generation of pluripotent stem-cell lines from almost any somatic tissue and mammalian species, thereby avoiding the ethical i...
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Chapter
Keratinocyte-Induced Pluripotent Stem Cells: From Hair to Where?
The generation of induced pluripotent stem (iPS) cells has spawned unprecedented opportunities for investigating the molecular mechanisms that underlie cellular pluripotency and reprogramming, as well as for o...
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Article
Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2
Induced pluripotent stem cells (iPSC) provide an invaluable resource for regenerative medicine as they allow the generation of patient-specific progenitors with potential value for cell therapy. However, in ma...
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Article
A protocol describing the genetic correction of somatic human cells and subsequent generation of iPS cells
The generation of patient-specific induced pluripotent stem cells (iPSCs) offers unprecedented opportunities for modeling and treating human disease. In combination with gene therapy, the iPSC technology can b...
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Article
Zebrafish heart regeneration occurs by cardiomyocyte dedifferentiation and proliferation
Zebrafish are able to efficiently regenerate lost cardiac muscle, and is used as a model to understand why natural heart regeneration is blocked in mammals. Two groups reporting in the issue of Nature used geneti...
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Article
Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells
The ease of generating induced pluripotent stem (iPS) cells, and possibly their properties after reprogramming, depends on the origin of the somatic cell starting population. Reprogramming of keratinocytes is ...
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Article
Open AccessRegeneration and reprogramming compared
Dedifferentiation occurs naturally in mature cell types during epimorphic regeneration in fish and some amphibians. Dedifferentiation also occurs in the induction of pluripotent stem cells when a set of transc...
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Article
Induced pluripotent stem cells and reprogramming: seeing the science through the hype
Four leading researchers contribute their personal opinions on recent progress and current challenges in induced pluripotent stem cell research. Their varied perspectives suggest that for clinical applications...
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Article
Linking the p53 tumour suppressor pathway to somatic cell reprogramming
This paper reports that the transcription factor p53 plays a crucial role in regulating somatic reprogramming. The authors develop an experimental protocol to reprogram somatic cells using just two factors, Oc...
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Article
Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells
The generation of induced pluripotent stem (iPS) cells has enabled the derivation of patient-specific pluripotent cells and provided valuable experimental platforms to model human disease. Patient-specific iPS...
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Protocol
Subtractive Hybridization and Construction of cDNA Libraries
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Chapter
Maintenance of Embryonic Stem Cell Pluripotency by Nanog-Mediated Dedifferentiation of Committed Mesoderm Progenitors
Embryonic stem (ES) cells can be propagated indefinitely in culture while retaining the ability to differentiate into any cell type in the organism. The molecular and cellular mechanisms underlying ES cell plu...
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Article
The forkhead protein Foxj1 specifies node-like cilia in Xenopus and zebrafish embryos
Chris Kintner and colleagues report that a Xenopus homolog of the transcription factor Foxj1 is sufficient to induce motile cilia by upregulating expression of genes encoding key components of the motile ciliary ...
