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Article
Cardiac fibroblasts and mechanosensation in heart development, health and disease
The term ‘mechanosensation’ describes the capacity of cells to translate mechanical stimuli into the coordinated regulation of intracellular signals, cellular function, gene expression and epigenetic programmi...
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Article
Open AccessPublic Cord Blood Banks as a source of starting material for clinical grade HLA-homozygous induced pluripotent stem cells
The increasing number of clinical trials for induced pluripotent stem cell (iPSC)-derived cell therapy products makes the production on clinical grade iPSC more and more relevant and necessary. Cord blood bank...
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Protocol
Porcine iPSC Generation: Testing Different Protocols to a Successful Application
Stem cell therapy has an unparalleled potential to treat blood cancers, cardiovascular diseases and neurodegenerative conditions, among others. However, stem cell therapeutics must overcome multiple requiremen...
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Article
Open AccessAtypical cyclin P regulates cancer cell stemness through activation of the WNT pathway
Cancer stem cells represent a cancer cell subpopulation that has been found to be associated with metastasis and chemoresistance. Therefore, it is vital to identify mechanisms regulating cancer stemness. Previ...
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Article
Open AccessInborn errors of metabolism: Lessons from iPSC models
The possibility of reprogramming human somatic cells to pluripotency has opened unprecedented opportunities for creating genuinely human experimental models of disease. Inborn errors of metabolism (IEMs) const...
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Article
Open AccessEvaluation of the Spanish population coverage of a prospective HLA haplobank of induced pluripotent stem cells
iPSC (induced pluripotent stem cells) banks of iPSC lines with homozygous HLA (human leukocyte antigen) haplotypes (haplobanks) are proposed as an affordable and off-the-shelf approach to allogeneic transplant...
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Article
Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
Epithelial repair and regeneration are driven by collective cell migration and division. Both cellular functions involve tightly controlled mechanical events, but how physical forces regulate cell division in ...
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Article
Open AccessWhole-genome DNA hyper-methylation in iPSC-derived dopaminergic neurons from Parkinson’s disease patients
Parkinson’s disease (PD) is characterized by the loss of midbrain dopaminergic neurons (DAn). Previously, we described the presence of DNA hyper- and hypo-methylation alterations in induced pluripotent stem ce...
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Article
Open AccessCRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons
Patient-specific induced pluripotent stem cells (iPSCs) are a powerful tool to investigate the molecular mechanisms underlying Parkinson’s disease (PD), and might provide novel platforms for systematic drug sc...
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Article
Using enhanced number and brightness to measure protein oligomerization dynamics in live cells
Protein dimerization and oligomerization are essential to most cellular functions, yet measurement of the size of these oligomers in live cells, especially when their size changes over time and space, remains ...
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Article
Open AccessCorrection to: The Small GTPase RAC1/CED-10 Is Essential in Maintaining Dopaminergic Neuron Function and Survival Against α-Synuclein-Induced Toxicity
With the author(s)’ decision to opt for Open Choice the copyright of the article changed on March 2018 to © The Author(s) 2018 and the name of one of the author was changed to “Sanjib Guha”.
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Article
Open AccessThe Small GTPase RAC1/CED-10 Is Essential in Maintaining Dopaminergic Neuron Function and Survival Against α-Synuclein-Induced Toxicity
Parkinson’s disease is associated with intracellular α-synuclein accumulation and ventral midbrain dopaminergic neuronal death in the Substantia Nigra of brain patients. The Rho GTPase pathway, mainly linking sur...
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Article
Prostaglandin EP2 Receptors Mediate Mesenchymal Stromal Cell-Neuroprotective Effects on Dopaminergic Neurons
Mesenchymal stromal cells (MSCs) have been shown to have useful properties for cell therapy and have been proposed for treatment of neurodegenerative diseases, including Parkinson’s disease. However, the mecha...
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Article
Open AccessiPS Cell Cultures from a Gerstmann-Sträussler-Scheinker Patient with the Y218N PRNP Mutation Recapitulate tau Pathology
Gerstmann-Sträussler-Scheinker (GSS) syndrome is a fatal autosomal dominant neurodegenerative prionopathy clinically characterized by ataxia, spastic paraparesis, extrapyramidal signs and dementia. In some GSS...
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Chapter
Pluripotent Stem Cell Banks
Embryonic stem cells (ESCs) are pluripotent stem cells that can be obtained from the inner cell mass (ICM) of blastocyst-stage pre-implantation embryos. The first lines of human ESC (hESC) were derived by Tho...
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Article
Open AccessLong-term in vivo single-cell lineage tracing of deep structures using three-photon activation
Genetic labeling techniques allow for noninvasive lineage tracing of cells in vivo. Two-photon inducible activators provide spatial resolution for superficial cells, but labeling cells located deep within tissues...
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Article
Open AccessEarly ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming
During the process of reprogramming to induced pluripotent stem (iPS) cells, somatic cells switch from oxidative to glycolytic metabolism, a transition associated with profound mitochondrial reorganization. Ne...
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Article
Open AccessNeoinnervation and neovascularization of acellular pericardial-derived scaffolds in myocardial infarcts
Engineered bioimplants for cardiac repair require functional vascularization and innervation for proper integration with the surrounding myocardium. The aim of this work was to study nerve sprouting and neovas...
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Article
Interplay of LRRK2 with chaperone-mediated autophagy
This study shows that Parkinson's disease–associated mutant forms of leucine-rich repeat kinase 2 (LRRK2) impair chaperone-mediated autophagy in neurons, thereby reducing degradation of α-synuclein by this pat...
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Article
Derivation of human embryonic stem cells at the Center of Regenerative Medicine in Barcelona
We report here the legislative issues related to embryo research and human embryonic stem cell (hESC) research in Spain and the derivation of nine hESC lines at the Center of Regenerative Medicine in Barcelona...
