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Article
Open AccessEarly stress exposure on zebrafish development: effects on survival, malformations and molecular alterations
The effects of stress during early vertebrate development can be especially harmful. Avoiding stressors in fish larvae is essential to ensure the health of adult fish and their reproductive performance and ove...
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Article
Open AccessImpact of different levels of handling on Solea senegalensis culture: effects on growth and molecular markers of stress
Aquaculture routine practices may cause stress induction on the fish and compromise their welfare affecting the production. This experiment aimed to evaluate the potential links between handling during culture...
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Article
Open AccessStress decreases spermatozoa quality and induces molecular alterations in zebrafish progeny
Chronic stress can produce a severe negative impact on health not only in the exposed individuals but also in their offspring. Indeed, chronic stress may be contributing to the current worldwide scenario of in...
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Protocol
In Vitro Induction of Teleost PGCs
are unique cells in an . These cells contain all genetic information and therefore represent the best source to store maternal and paternal genomes until is achieved. However, the number of these cells i...
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Protocol
Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa
Reactive oxygen species (ROS) could have a negative impact on sperm cellular function and viability. This chapter describes a protocol for oxidative stress evaluation using dichlorofluorescein (DCF) which can ...
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Article
Open AccessEffect of low sperm quality on progeny: a study on zebrafish as model species
Nowadays a decrease tendency in human sperm quality has been reported mainly in developed countries. Reproductive technologies have been very valuable in achieving successful pregnancies with low quality sperm...
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Protocol
Chapter 19 Cryopreservation Effect on Genetic Function: Neonatal Outcomes
Cryopreservation is a well-established technique commonly used in clinical practice. It is used widely for the conservation of gametes and embryos that will be used later for insemination or in vitro fertiliza...
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Protocol
Flow Cytometric Methods for Sperm Assessment
Flow cytometry allows the assessment of multiple sperm parameters following a diverse number of protocols. Here, we describe three methods for evaluating critical aspects of sperm quality: the double stain SYB...
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Article
Open AccessRegeneration and reprogramming compared
Dedifferentiation occurs naturally in mature cell types during epimorphic regeneration in fish and some amphibians. Dedifferentiation also occurs in the induction of pluripotent stem cells when a set of transc...
