-
Article
Open AccessFocal adhesions contain three specialized actin nanoscale layers
Focal adhesions (FAs) connect inner workings of cell to the extracellular matrix to control cell adhesion, migration and mechanosensing. Previous studies demonstrated that FAs contain three vertical layers, wh...
-
Article
Ligand-dependent localization and function of ORP–VAP complexes at membrane contact sites
Oxysterol-binding protein/OSBP-related proteins (ORPs) constitute a conserved family of sterol/phospholipid-binding proteins with lipid transporter or sensor functions. We investigated the spatial occurrence a...
-
Protocol
Kinetic Activation of Rab8 Guanine Nucleotide Exchange Factor Rabin8 by Rab11
The Rab family of small GTPases acts as molecular switches that control various stages of vesicular transport. Rab8 functions in exocytic trafficking from the trans-Golgi network (TGN) and recycling endosomes to ...
-
Article
Open AccessTristetraprolin is a novel regulator of BDNF
Brain-derived neurotrophic factor (BDNF) regulates multiple biological processes ranging from central nervous system development and function to neuroinflammation and myogenic differentiation and repair. While...
-
Article
Pinkbar is an epithelial-specific BAR domain protein that generates planar membrane structures
A previously uncharacterized member of the I-BAR subfamily of BAR-domain proteins, Pinkbar, is expressed in epithelial cells, where it stabilizes planar membrane sheets. Structural, biochemical and mutagenesis...
-
Article
A molecular network for de novo generation of the apical surface and lumen
Polarized-membrane trafficking supports the delivery of polarity proteins to discrete plasma-membrane domains, although the interplay between trafficking and polarity pathways is not fully understood. The smal...
-
Article
1H, 13C and 15N resonance assignments of the human mesencephalic astrocyte-derived neurotrophic factor
Parkinson’s disease (PD) is a chronic, progressive neurodegenerative disease, where dopaminergic cells die most prominently in the area of substantia nigra. Neurotrophic factors (NTFs) are secreted proteins, w...
-
Article
Novel neurotrophic factor CDNF protects and rescues midbrain dopamine neurons in vivo
The drugs currently available for Parkinson's disease patients generally treat only the symptoms, and do not prevent the degeneration and death of dopaminergic neurons in the brain, the cause the motor defects...
-
Article
N-glycans as apical sorting signals in epithelial cells
IN epithelial Madin–Darby canine kidney (MDCK) cells newly synthesized molecules are sorted in the trans-Golgi network and directly delivered to their apical and basolateral surface destinations1. Sorting is medi...