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Article
Open AccessTraject3d allows label-free identification of distinct co-occurring phenotypes within 3D culture by live imaging
Single cell profiling by genetic, proteomic and imaging methods has expanded the ability to identify programmes regulating distinct cell states. The 3-dimensional (3D) culture of cells or tissue fragments prov...
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Protocol
The Use of Three-Dimensional Cell Culture to Study Apicobasal Polarization and Lumen Formation
The three-dimensional culture of epithelial cells allows the characterization of processes required for collective epithelial polarization, such as formation of an epithelial lumen. Madin-Darby Canine Kidney (...
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Article
Open AccessAn ARF GTPase module promoting invasion and metastasis through regulating phosphoinositide metabolism
The signalling pathways underpinning cell growth and invasion use overlap** components, yet how mutually exclusive cellular responses occur is unclear. Here, we report development of 3-Dimensional culture an...
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Article
Open AccessThe phospholipid PI(3,4)P2 is an apical identity determinant
Apical-basal polarization is essential for epithelial tissue formation, segregating cortical domains to perform distinct physiological functions. Cortical lipid asymmetry has emerged as a determinant of cell p...
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Article
Open AccessA functional genomics screen reveals a strong synergistic effect between docetaxel and the mitotic gene DLGAP5 that is mediated by the androgen receptor
Based on a molecular classification of prostate cancer using gene expression pathway signatures, we derived a set of 48 genes in critical pathways that significantly predicts clinical outcome in all tested pat...
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Article
Fam49/CYRI interacts with Rac1 and locally suppresses protrusions
Actin-based protrusions are reinforced through positive feedback, but it is unclear what restricts their size, or limits positive signals when they retract or split. We identify an evolutionarily conserved reg...
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Article
Role of membrane traffic in the generation of epithelial cell asymmetry
Epithelial cells have an apical–basolateral axis of polarity, which is required for epithelial functions including barrier formation, vectorial ion transport and sensory perception. Here we review what is know...
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Article
Synaptotagmin-like proteins control the formation of a single apical membrane domain in epithelial cells
The formation of epithelial tissues requires both the generation of apical–basal polarity and the coordination of this polarity between neighbouring cells to form a central lumen. During de novo lumen formation, ...
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Article
A kinase cascade leading to Rab11-FIP5 controls transcytosis of the polymeric immunoglobulin receptor
Epithelial cell transcytosis of polymeric IgA by its receptor requires polarized membrane trafficking. A signalling cascade involving the tyrosine kinase YES, EGFR, ERK and the Rab11 effector FIP5 is now shown...
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Article
A molecular network for de novo generation of the apical surface and lumen
Polarized-membrane trafficking supports the delivery of polarity proteins to discrete plasma-membrane domains, although the interplay between trafficking and polarity pathways is not fully understood. The smal...
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Article
From cells to organs: building polarized tissue
Most cells in the body are polarized, showing some level of shape and functional asymmetry, such as apico–basal or front–back polarity.
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Article
Woody debris contribution to the carbon budget of selectively logged and maturing mid-latitude forests
Woody debris (WD) is an important component of forest C budgets, both as a C reservoir and source of CO2 to the atmosphere. We used an infrared gas analyzer and closed dynamic chamber to measure CO2 efflux from d...
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Article
Forest community analysis and the point-centered quarter method
The point-centered-quarter (PCQ) method has been applied in community analysis since the publication of the method nearly 50 years ago. This and other distance methods offer increased sampling efficiency over ...
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Article
Zebra finches, Taeniopygia guttata, with elongated tails delay taking flight during group take-offs
Zebra finches with tail-elongations left perches during induced take-offs consistently later than unmanipulated controls. Latency in take-off was the same for both a short pintail-elongation (27% of finch long...
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Article
Climate change and constraints on breeding
Although climate change apparently affects the breeding patterns of many animals1,2,3, the wider implications for breeding success are unclear. Here we describe an energy trade-off between reproduction and mainte...
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Article
Energetics of free existence in swallows and martins (hirundinidae) during breeding: a comparative study using doubly labeled water
Energy metabolism of three sympatric swallows (Hirundinidae) was investigated during the breeding season using doubly labeled water (2H2 18O). Interspecific and intraspecific differences in energy metabolism were...
