42 Result(s)

Article

Nanobody-induced perturbation of LFA-1/L-plastin phosphorylation impairs MTOC docking, immune synapse formation and T cell activation

The T cell integrin receptor LFA-1 orchestrates adhesion between T cells and antigen-presenting cells (APCs), resulting in formation of a contact zone known as the immune synapse (IS) which is supported by the...

Sarah De Clercq, Olivier Zwaenepoel… in Cellular and Molecular Life Sciences (2013)

Article

Selecting protein N-terminal peptides by combined fractional diagonal chromatography

In recent years, procedures for selecting the N-terminal peptides of proteins with analysis by mass spectrometry have been established to characterize protease-mediated cleavage and protein α-N-acetylation on a p...

An Staes, Francis Impens, Petra Van Damme, Bart Ruttens, Marc Goethals… in Nature Protocols (2011)

Article

Combining quantitative proteomics data processing workflows for greater sensitivity

An algorithm to combine the results of different quantitative proteomics data processing workflows is presented. The method substantially increases the number of proteins that can be quantified in a proteomics...

Niklaas Colaert, Christophe Van Huele, Sven Degroeve, An Staes… in Nature Methods (2011)

Protocol

Mass Spectrometry-Driven Proteomics: An Introduction

Proteins are reckoned to be the key actors in a living organism. By studying proteins, one engages into deciphering a complex series of events occurring during a protein’s life span. This starts at the creatio...

Kenny Helsens, Lennart Martens, Joël Vandekerckhove, Kris Gevaert in Gel-Free Proteomics (2011)

Protocol

A Case Study on the Comparison of Different Software Tools for Automated Quantification of Peptides

MS-driven proteomics has evolved over the past two decades to a high tech and high impact research field. Two distinct factors clearly influenced its expansion: the rapid growth of an arsenal of instrument and...

Niklaas Colaert, Joël Vandekerckhove, Lennart Martens, Kris Gevaert in Gel-Free Proteomics (2011)

Article

Complementary positional proteomics for screening substrates of endo- and exoproteases

A method to simultaneously analyze C- and N-terminal peptides using a combination of strong cation exchange, diagonal chromatography and mass spectrometry is described, allowing the screening of protease subst...

Petra Van Damme, An Staes, Silvia Bronsoms, Kenny Helsens… in Nature Methods (2010)

Article

A llama-derived gelsolin single-domain antibody blocks gelsolin–G-actin interaction

RNA interference has tremendously advanced our understanding of gene function but recent reports have exposed undesirable side-effects. Recombinant Camelid single-domain antibodies (VHHs) provide an attractive me...

Anske Van den Abbeele, Sarah De Clercq… in Cellular and Molecular Life Sciences (2010)

Article

Listeria monocytogenes impairs SUMOylation for efficient infection

During infection the food-borne pathogen Listeria monocytogenes exploits a large number of the host cell's functions including post-translational modifications involving ubiquitinylation and phosphorylation, whic...

David Ribet, Mélanie Hamon, Edith Gouin, Marie-Anne Nahori, Francis Impens… in Nature (2010)

Article

The PDZ2 domain of zonula occludens-1 and -2 is a phosphoinositide binding domain

Zonula occludens proteins (ZO) are postsynaptic density protein-95 discs large-zonula occludens (PDZ) domain-containing proteins that play a fundamental role in the assembly of tight junctions and establishmen...

Kris Meerschaert, Moe Phyu Tun, Eline Remue… in Cellular and Molecular Life Sciences (2009)

Article

Improved visualization of protein consensus sequences by iceLogo

Niklaas Colaert, Kenny Helsens, Lennart Martens, Joël Vandekerckhove… in Nature Methods (2009)

Article

Analysis of the γ-secretase interactome and validation of its association with tetraspanin-enriched microdomains

The γ-secretase complex is responsible for the generation of amyloid β-peptide, the main component of Alzheimer's disease associated plaques. A proteomic analysis yielded secretory pathway components and membr...

Tomoko Wakabayashi, Katleen Craessaerts, Leen Bammens… in Nature Cell Biology (2009)

Protocol

Reverse-Phase Diagonal Chromatography for Phosphoproteome Research

We present a gel-free proteomics procedure for the specific isolation of phosphorylated peptides from whole proteome digests. Central is the use of diagonal, reverse-phase chromatography which consists of two ...

Kris Gevaert, Joël Vandekerckhove in Phospho-Proteomics (2009)

Protocol

Map** protein N-Glycosylation by COFRADIC

The majority of proteins require co- and posttranslational modifications to become functional entities. Such modifications fulfill a plethora of tasks (e.g., modulation of enzyme activity and directing protein...

Bart Ghesquière, Joël Vandekerckhove, Kris Gevaert in The Protein Protocols Handbook (2009)

Article

Human Proteinpedia enables sharing of human protein data

Suresh Mathivanan, Mukhtar Ahmed, Natalie G Ahn, Hainard Alexandre… in Nature Biotechnology (2008)