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Protocol
Analysis of Invasion Dynamics of Matrix-Embedded Cells in a Multisample Format
In vitro tests of cancer cell invasion are the “first line” tools of preclinical researchers for screening the multitude of chemical compounds or cell perturbations that may aid in halting or treating cancer m...
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Article
Open AccessIdentification and expression analysis of splice variants of mouse enabled homologue during development and in adult tissues
The Enabled/Vasodilator stimulated phosphoprotein (Ena/VASP) gene family comprises three genes in vertebrates: Vasp, Enabled homologue (Enah) and Ena-VASP like (Evl). Enah has the most complex gene structure. It ...
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Article
Open AccessPhenotypes induced by NM causing α-skeletal muscle actin mutants in fibroblasts, Sol 8 myoblasts and myotubes
Nemaline myopathy is a neuromuscular disorder characterized by the presence of nemaline bodies in patient muscles. 20% of the cases are associated with α-skeletal muscle actin mutations. We previously showed t...
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Article
A method for rapidly screening functionality of actin mutants and tagged actins
Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state. We therefore ha...
