31 Result(s)

Chapter

Development of Microbial Sensors and Their Application

Many types of microbial sensors have been developed as analytical tools since the first microbial sensor was studied by Karube et al. in 1977. The microbial sensor consists of a transducer and microbe ...

Hideaki Nakamura, Mifumi Shimomura-Shimizu, Isao Karube in Biosensing for the 21st Century (2008)

Article

Current research activity in biosensors

Biosensors consist of a molecular recognition element and a transducer. Since the first biosensor was developed by Updike and Hicks [1] many biosensors and their associated techniques have been studied and dev...

Hideaki Nakamura, Isao Karube in Analytical and Bioanalytical Chemistry (2003)

Article

Fructose-2,6-bisphosphate contents were increased in response to salt, water and osmotic stress in leaves of Bruguiera gymnorrhiza by differential changes in the activity of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphate 2-phosphatase

The steady-state level of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphate 2-phosphatase (F6P2K/F26BPase) transcript has been found to be raised in the mangrove Bruguiera gymnorrhiza treated with 500 mM NaCl fo...

Toshiaki Banzai, Nobutaka Hanagata, Zvy Dubinsky, Isao Karube in Plant Molecular Biology (2003)

Article

Detection technique of asymmetric RT-PCR-based amplified single-stranded DNA and its application to biosensor for detection of mRNA for cyanobacteria, Anabaena variabilis

A real-time detection technique for mRNA was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the expressed mRNA of Anabaena variabilis PCC 7120 by using t...

Ryoichi Asai, Chikashi Nakamura, Kazunori Ikebukuro, Isao Karube… in Biotechnology Letters (2002)

Article

Isolation of a bacterium from mangrove soil for degradation of sea sludge

Sea sludge, which is sediment of fish excrement and sewage on the sea bottom, continues to be a serious environmental problem. It has the potential to cause eutrophication and red tide, resulting in the death ...

Yoshihiro Ando, Naoya Mitsugi, Kazuyoshi Yano… in Applied Biochemistry and Biotechnology (2001)

Article

Eicosapentaenoic acid release from the red alga Pachymeniopsis lanceolata by enzymatic degradation

Forty-eight species of seaweeds from Japanese waters were screened for the valuable polyunsaturated fatty acids eicosapentaenoic acid (EPA). The eight species that contained the highest levels of these compoun...

Ritsuko Matsukawa, Kiyotaka Hatakeda, Shota Ito… in Applied Biochemistry and Biotechnology (1999)

Protocol

Microbial biosensors based on respiratory inhibition

Biosensors generally provide a rapid and convenient alternative to conventional methods for monitoring chemical substances in fields as diverse as medicine, environmental monitoring, and food processing. A bio...

Yoshiko Arikawa, Kazunori Ikebukuro, Isao Karube in Enzyme and Microbial Biosensors (1998)

Article

Enzymatic screening of microalgae as a potential source of natural antioxidants

Extracts of over 100 species of microalgae both from nature and from laboratory cultures were screened for antioxidant activity. As an assay for antioxidant function, we examined the inhibition of the activity...

Ritsuko Matsukawa, Zvy Dubinsky, Kazuyoshi Masaki… in Applied Biochemistry and Biotechnology (1997)

Article

Determination of cyanide using a microbial sensor

A microbial cyanide sensor was prepared, consisting of immobilizedSaccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of ...

Keijiro Nakanishi, Kazunori Ikebukuro… in Applied Biochemistry and Biotechnology (1996)

Article

A novel synthesis method for cyclodextrins from maltose in water-organic solvent systems

A novel enzymatic synthesis method of cyclodextrin (CD) from low-mol-wt maltose using cyclomaltodextrin glucanotransferase (CGTase) fromBacillus macerans has been developed in various water-organic solvent system...

Takashi Morita, Nobuyuki Yoshida, Isao Karube in Applied Biochemistry and Biotechnology (1996)

Article

Enzymatic hydrolysis of starch in water-immiscible organic solvent, two-phase systems

Enzyme-catalyzed hydrolyzations of starch by α-amylase have been studied in various two-phase systems, consisting of water and a water-immiscible organic solvent. The hydrolytic conversion of soluble starch to...

Takashi Morita, Isao Karube in Applied Biochemistry and Biotechnology (1995)

Article

Continuous two step bioconversion of 4-oxoiso-phorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclo-hexanone (4-HTMCH) by thermophilic bacteria

The continuous two step conversion of 4-oxoisophorone(OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone(4-HTMCH) via dihydrooxoisophorone(DOIP) was carried out using two types of thermophilic growing cells, Thermomo...

Kunio Nishii, Koji Sode, Isao Karube in Biotechnology Letters (1995)

Article

Influence of cationic antibiotics on phase behavior of rough-form lipopolysaccharide

The rough-form lipopolysaccharide (LPS) interacted with cationic antibiotic polymyxin B and gramicidin S in solution, and showed altered thermotropic phase behavior and viscoelasticity. The phase behavior was ...

Satoshi Fukuoka, Isao Karube in Applied Biochemistry and Biotechnology (1994)

Article

Research and development of biosensors in Japan

Isao Karube in Applied Biochemistry and Biotechnology (1993)

Chapter

Microbial Sensors and Microbiosensors

A novel BOD sensor with a new principle employing a luminous bacterium, Photobacterium phosphoreum, was developed. The increased intensity of luminescence resulting from the cellular assimilation of organic compo...

Isao Karube, Kenji Yokoyama in Uses of Immobilized Biological Compounds (1993)

Article

A biocompatible needle-type glucose sensor based on platinum-electroplated carbon electrode

A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a d...

Chien-Yuan Chen, Eiichi Tamiya, Kazuhiko Ishihara… in Applied Biochemistry and Biotechnology (1992)

Article

Structural characterization of lipid A component of Erwinia carotovora lipopolysaccharide

The chemical structure of the lipid A component of lipopolysaccharide excreted into the liquid medium by the plant pathogenic enterobacterium Erwinia carotovora FERM P-7576 was characterized. It consists of a β-1...

Satoshi Fukuoka, Hiroshi Kamishima, Yoshinobu Nagawa… in Archives of Microbiology (1992)

Article

Amplification immunoassay for the determination of Hepatitis B surface antigen

A sensitive sandwich immunoassay for the determination of Hepatitis B surface antigen (HBs) was developed, using a cascade system of Limulus amebocyte lysate as a signal amplification system. Lipo-polysacchari...

Atsushi Seki, Eiichi Tamiya, Isao Karube in Applied Biochemistry and Biotechnology (1991)

Article

Facile isolation ofEndo-pectate lyase fromErwinia carotovora based on electrostatic interaction

Endo-pectate lyase (PATE) fromErwinia carotovora was selectively cosedimented with extracellularly produced lipopolysaccharide-lipid complex (LPSLC) through dialysis of the cell free culture broth. The selective ...

Satoshi Fukuoka, Hiroshi Kamishima, Koji Sode… in Applied Biochemistry and Biotechnology (1990)