-
Article
Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells
Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use...
-
Article
Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511
The development of fully defined culture conditions for human embryonic stem cells (hESCs) should enhance experimental reproducibility, reduce unwanted contaminants and facilitate scale-up production. Rodin et al
-
Article
Open AccessClonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment
Lack of robust methods for establishment and expansion of pluripotent human embryonic stem (hES) cells still hampers development of cell therapy. Laminins (LN) are a family of highly cell-type specific basemen...
-
Article
Open AccessManipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells
Human pluripotent stem cell (hPSC)-derived endothelial lineage cells constitutes a promising source for therapeutic revascularization, but progress in this arena has been hampered by a lack of clinically-scala...
-
Article
Erratum: Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells
Correction to: Cell Research (2014) 24:820–841. doi:10.1038/cr.2014.59; published online 9 May 2014 The protocol in the current study was developed using BIO as the GSK-3β inhibitor and works with other inhibi...
-
Article
Open AccessMultiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology
Cells typically respond to chemical or physical perturbations via complex signaling cascades which can simultaneously affect multiple physiological parameters, such as membrane voltage, calcium, pH, and redox ...
-
Article
Open AccessSingle-cell analysis uncovers fibroblast heterogeneity and criteria for fibroblast and mural cell identification and discrimination
Many important cell types in adult vertebrates have a mesenchymal origin, including fibroblasts and vascular mural cells. Although their biological importance is undisputed, the level of mesenchymal cell heter...
-
Article
Open AccessPublisher Correction: Single-cell analysis uncovers fibroblast heterogeneity and criteria for fibroblast and mural cell identification and discrimination
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
-
Article
Open AccessLeft ventricular hypertrophy and metabolic resetting in the Notch3-deficient adult mouse heart
The heart depends on a functional vasculature for oxygenation and transport of nutrients, and it is of interest to learn how primary impairment of the vasculature can indirectly affect cardiac function and hea...
