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Open AccessAnalysis of local extracellular matrix identifies different aetiologies behind bicuspid and tricuspid aortic valve degeneration and suggests therapies
Aortic valve degeneration (AVD) is a life-threatening condition that has no medical treatment and lacks individual therapies. Although extensively studied with standard approaches, aetiologies behind AVD are u...
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Open AccessAn integrative proteomics method identifies a regulator of translation during stem cell maintenance and differentiation
Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. To systematically study s...
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Open AccessSystem-wide identification and prioritization of enzyme substrates by thermal analysis
Despite the immense importance of enzyme–substrate reactions, there is a lack of general and unbiased tools for identifying and prioritizing substrate proteins that are modified by the enzyme on the structural...
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Article
Open AccessIsotopic resonance at 370 ppm deuterium negatively affects kinetics of luciferin oxidation by luciferase
Since 1930s, it has been known that some biochemical and biological processes exhibit abnormal kinetics at a deuterium concentration in the local environment of 250–600 ppm, which is 2–4 times higher that the ...
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Article
Open AccessCumulative prognostic power of laminin genes in colorectal cancer
Laminins are a major family of extracellular matrix proteins and the main component of basement membranes. Laminins are involved in many if not all stages of cancer progression, and expression of laminin genes...
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Biologically Relevant Laminins in Regenerative Medicine
Need for biologically relevant . Recent advances in -based allow to expand human in almost unlimited amounts. However, expanded become devoid of natural niche cues and, therefore, may become prone to risk...
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Monolayer culturing and cloning of human pluripotent stem cells on laminin-521–based matrices under xeno-free and chemically defined conditions
Coating of cell culture plates with laminin-521 allows culturing of human pluripotent stem cells and, in combination with E-cadherin, their cloning in individual wells.
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Article
Open AccessClonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment
Lack of robust methods for establishment and expansion of pluripotent human embryonic stem (hES) cells still hampers development of cell therapy. Laminins (LN) are a family of highly cell-type specific basemen...
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Article
Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511
The development of fully defined culture conditions for human embryonic stem cells (hESCs) should enhance experimental reproducibility, reduce unwanted contaminants and facilitate scale-up production. Rodin et al
