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Article
Co-localization of NANOG and OCT4 in human pre-implantation embryos and in human embryonic stem cells
NANOG and OCT4 are required for the maintenance of pluripotency in embryonic stem cells (ESCs). These proteins are also expressed in the inner cell mass (ICM) of the mouse pre-implantation embryo.
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Protocol
Xeno-Free Culture of Human Pluripotent Stem Cells
Stem cell culture systems that rely on undefined animal-derived components introduce variability to the cultures and complicate their therapeutic use. The derivation of human embryonic stem cells and the devel...
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Article
Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511
The development of fully defined culture conditions for human embryonic stem cells (hESCs) should enhance experimental reproducibility, reduce unwanted contaminants and facilitate scale-up production. Rodin et al
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Article
Open AccessDerivation of 30 human embryonic stem cell lines—improving the quality
We have derived 30 human embryonic stem cell lines from supernumerary blastocysts in our laboratory. During the derivation process, we have studied new and safe method to establish good quality lines. All our ...