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Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells

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Abstract

Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describes an efficient one-step deglycosylation method using gaseous hydrogen fluoride on nylon blotting membranes and microtiter wells.

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Authors and Affiliations

  1. Department of Medical Biochemistry, University of Goteborg, Medicinaregatan 9, 413 90, Gothenburg, Sweden

    Gunnar C Hansson

Authors
  1. Magnus A.B Axelsson
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  2. Emil M Hansson
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  3. Rein Sikut
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  4. Gunnar C Hansson
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Axelsson, M.A., Hansson, E.M., Sikut, R. et al. Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells. Glycoconj J 15, 749–755 (1998). https://doi.org/10.1023/A:1006935227730

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  • DOI: https://doi.org/10.1023/A:1006935227730

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