-
Article
Open AccessAn efficient approach to finding Siraitia grosvenorii triterpene biosynthetic genes by RNA-seq and digital gene expression analysis
Siraitia grosvenorii (Luohanguo) is an herbaceous perennial plant native to southern China and most prevalent in Guilin city. Its fruit contains a sweet, fleshy, edible pulp that is widely used in traditional Chi...
-
Article
Open AccessDe novo assembly of Euphorbia fischeriana root transcriptome identifies prostratin pathway related genes
Euphorbia fischeriana is an important medicinal plant found in Northeast China. The plant roots contain many medicinal compounds including 12-deoxyphorbol-13-acetate, commonly known as prostratin that is a phorbo...
-
Article
De novo sequencing and a comprehensive analysis of purple sweet potato (Impomoea batatas L.) transcriptome
High-throughput RNA sequencing was performed for comprehensively analyzing the transcriptome of the purple sweet potato. A total of 58,800 unigenes were obtained and ranged from 200 nt to 10,380 nt with an ave...
-
Article
Open AccessGenome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431
Paclitaxel (Taxol™) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae,...
-
Article
Open AccessDe novo transcriptome sequencing and digital gene expression analysis predict biosynthetic pathway of rhynchophylline and isorhynchophylline from Uncaria rhynchophylla, a non-model plant with potent anti-alzheimer’s properties
The major medicinal alkaloids isolated from Uncaria rhynchophylla (gouteng in chinese) capsules are rhynchophylline (RIN) and isorhynchophylline (IRN). Extracts containing these terpene indole alkaloids (TIAs) ca...
-
Chapter
Genetics and Breeding of Danshen
Genuine Danshen medicinal material comes from the dry and talks of Danshen, a Labiatae plant. With decreasing wild resources, the supply of medicinal Danshen is already unable to meet the demand. Presently, mo...
-
Chapter
Danshen’s In Vitro Culture
Cultivated Danshen tends to have low contents of effective and uneven quality, and long-term asexual can easily lead to degeneration. Due to soil pollution by pesticides, heavy metals, and other pollutants i...
-
Article
Open AccessComparative analysis of the Dicer-like gene family reveals loss of miR162 target site in SmDCL1 from Salvia miltiorrhiza
DCL1, the core component for miRNA biogenesis, is itself regulated by miR162 in Arabidopsis. MiRNA-mediated feedback regulation of AtDCL1 is important to maintain the proper level of DCL1 transcripts. However, it...
-
Article
Open AccessIdentification of novel and conserved microRNAs in Panax notoginseng roots by high-throughput sequencing
MicroRNAs (miRNAs) are small, non-coding RNAs that are important regulators of gene expression, and play major roles in plant development and their response to the environment. Root extracts from Panax notoginsen...
-
Article
Genome-wide analysis of the terpene synthase gene family in physic nut (Jatropha curcas L.) and functional identification of six terpene synthases
Plant terpenes constitute a large class of compounds and have numerous biological roles as either primary or secondary metabolites. Terpene synthases (TPSs) play key roles on catalyzing the formation of differ...
-
Article
Whole transcriptome analysis of three leaf stages in two cultivars and one of their F1 hybrid of Camellia sinensis L. with differing EGCG content
Tea (Camellia sinensis (L.) O. Kuntze) is one of the most beneficial beverages due to its health attributes. Whole leaf transcriptome analysis of three leaf stages of C. sinensis has the potential to reveal candi...
-
Chapter
Tissue Culture and Hairy Root Induction of Salvia miltiorrhiza
Salvia miltiorrhiza is a well-known medicinal plant with great economic and medicinal value. Its root contains two major groups of bioactive compounds, including lipophilic diterpenoid tanshinones and hydrophilic...
-
Article
Open AccessA comparative metabolomics analysis of the components of heartwood and sapwood in Taxus chinensis (Pilger) Rehd.
Taxus chinensis is a well-known gymnosperm with great ornamental and medicinal value. Its purple red brown heartwood (HW) has many attributes such as straight texture, high density, mechanical strength, rich elas...
-
Article
Open AccessSelection and validation of reference genes for quantitative gene expression normalization in Taxus spp.
Quantitative real-time PCR (qRT-PCR) is commonly used to measure gene expression to further explore gene function, while suitable reference genes must be stably expressed under different experimental condition...
-
Article
A comparative analysis of differential N6-methyladenosine (m6A) modification between non-transgenic and LBD15 overexpressing Poplar 84 K plants
N6-methyladenosine (m6A) plays an important role in the gene expression regulation. Previously, we found an ortholog of Arabidopsis LBD15 that showed xylem preferential expression and involved in leaf developm...
-
Article
Characterization and expression analysis of genes encoding Taxol biosynthetic enzymes in Taxus spp.
Taxol (Paclitaxel), an important anticancer drug, is derived at very low yields from Taxus (yew) species that grow very slowly. In the present study, thirteen genes that encode enzymes involved in Taxol biosynthe...
-
Article
Effects of ferrous sulfate and methyl jasmonate treatment on the content of 2-(2-phenethyl)chromones in Aquilaria sinensis
The 2-(2-phenylethyl) chromones are the main characteristic constituents for the fragrance and pharmacological activities of agarwood. Iron and methyl jasmonate (MeJA) are two important elicitors that affect t...
-
Article
Genome-wide identification of GATA transcription factor family and the effect of different light quality on the accumulation of terpenoid indole alkaloids in Uncaria rhynchophylla
Uncaria rhynchophylla is an evergreen vine plant, belonging to the Rubiaceae family, that is rich in terpenoid indole alkaloids (TIAs) that have therapeutic effects on hypertension and Alzheimer’s disease. GATA t...
