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Whole transcriptome analysis of three leaf stages in two cultivars and one of their F1 hybrid of Camellia sinensis L. with differing EGCG content

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Abstract

Tea (Camellia sinensis (L.) O. Kuntze) is one of the most beneficial beverages due to its health attributes. Whole leaf transcriptome analysis of three leaf stages of C. sinensis has the potential to reveal candidate genes associated with these major beneficial compounds. Epigallocatechin-3-gallate (EGCG) is one of the most important bioactive catechin (antioxidant) in tea drink derived. EGCG has many important physiological functions and positive effects on human health, such as anticancer, antioxidation, controlling blood pressure, and blood sugar content. Although the major metabolic pathways of tea-specific compounds have been established, knowledge of the genes involved in the regulation and synthesis of EGCG in C. sinensis is limited. To gain insight into the molecular mechanisms that regulate EGCG levels, leaf transcriptomes were sequenced at three developmental stages (bud, second leaf, and fourth leaf) of two C. sinensis cultivars and one F1 hybrid line #1005 that possess varying EGCG levels. Approximately 470.9 M of quality controlled-filtered sequencing paired-end reads corresponding to 47.09 G nucleotides were obtained from which 130,762 unique sequences were identified. Genes whose expression correlated with changes in EGCG levels were identified, which may be associated with EGCG content. Prediction of the potential targets of microRNAs (miRNAs), the specific subset of long noncoding RNAs (LncRNAs), and mRNA-like noncoding RNAs (mlncRNAs) indicated that miRNAs may play a role in the expression regulation of the genes involved in EGCG levels.

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Acknowledgments

This research was financially supported by the grants from the National Natural Science Foundation of China (grant no. 31170651). We thank Dr. Liangsheng Zhang for technical assistance and Li Zhang for critical reading of the manuscript.

Author information

Author notes

  1. Iain W. Wilson

    Present address: CSIRO Agriculture & Food, Canberra, ACT, Australia

  2. Guo** Ge

    Present address: Agricultural Extension Service Center, Dazhe, Suichang, Zejiang, China

Authors and Affiliations

  1. Department of Tea Science, Anxi College of Tea Science, Fujian Agriculture and Forestry University, Fuzhou, China

    **ke Lin, Guo** Ge, Liangyu Wu & Yue Zhang

  2. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China

    Guiling Sun & Jianqiang Wu

  3. Department of Biology, East Carolina University, Greenville, NC, USA

    Fuliang **e & Baohong Zhang

  4. State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Bei**g, China

    Yanfang Yang & Deyou Qiu

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  1. **ke Lin
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  2. Iain W. Wilson
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Corresponding author

Correspondence to Deyou Qiu.

Additional information

Communicated by J. L. Wegrzyn

**ke Lin, Iain W. Wilson, and Guo** Ge contributed equally to this work.

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Lin, J., Wilson, I.W., Ge, G. et al. Whole transcriptome analysis of three leaf stages in two cultivars and one of their F1 hybrid of Camellia sinensis L. with differing EGCG content. Tree Genetics & Genomes 13, 13 (2017). https://doi.org/10.1007/s11295-016-1089-5

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  • DOI: https://doi.org/10.1007/s11295-016-1089-5

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