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Article
Comparison of the Sorption Capacity of Different Brands of Activated Carbon Relative to Argon, Krypton, and Xenon with the Natural Isotopic Composition under Static Conditions
The Henry constants upon the adsorption of inert gases with a natural isotopic composition (argon, krypton, and xenon) on activated carbon of the AG-3, VSK-5, SKT-3, and NWC 12x40 brands in a temperature range...
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Article
Synthetic Biology: Current State and Applications
This review is devoted to synthetic biology. In this field, molecular biology methods and techniques are applied to develop a predetermined cell behavior based on theoretically constructed regulatory networks ...
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Article
Transcriptome Analysis of Signaling Pathways in Caco-2 Cells Involved in the Formation of Intestinal Villi
Caco-2 cells are traditionally used to construct in vitro models of the intestinal barrier. One characteristic of the mature intestine is the presence of villi—connective tissue outgrowths covered with epithel...
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Article
Development of Nanofiltration Ceramic Membrane Production Technology
The possibility of obtaining nanofiltration membranes by depositing metal-oxide nanopowders on ceramic substrates with macropores is discussed. Titanium, aluminum, and magnesium oxides obtained by the plasma t...
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Article
Modeling the Distribution of the Electromagnetic Field inside a Microcuvette Designed for Measuring the Impedance Spectra of Three-Dimensional Cell Cultures
Spectra obtained using a four-electrode measurement scheme in a medium with dispersion are modeled mathematically using the example of a three-dimensional cell culture. The problem is solved with the finite el...
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Article
Develo** a Microfluidic Device with an Integrated Electrode System for Measuring the Impedance Spectra of Cellular Models in Real Time
A mathematical model is presented that describes the distribution of the potential and electric field strength in cells of a microfluidic device for culturing a cellular monolayer with built-in electrodes of d...
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Article
Open AccessStorage and erasure of behavioural experiences at the single neuron level
Although predictions from the past about the future have been of major interest to current neuroscience, how past and present behavioral experience interacts at the level of a single neuron remains largely unk...
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Article
Impedance Spectroscopy and Transcriptome Analysis of Choriocarcinoma BeWo b30 as a Model of Human Placenta
Placenta is a highly specialized organ that is necessary for successful gestation. Several models of the placental barrier are used to study how it functions, including the transplacental transport of xenobiot...
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Article
Comparison of Profiles of Extracellular MicroRNA Secreted by Caco-2 Cells from the Apical Side of the Membrane under Static and Microcirculation Conditions
Extracellular microRNA are one of the indicators of the functional state of cells. Culturing of Caco-2 cells under the conditions of microcirculation in a Homunculus microfluidic device allows better simulatin...
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Article
Application of Impedance Spectroscopy for the Control of the Integrity of In Vitro Models of Barrier Tissues
We compared available methods for monitoring the integrity of in vitro models of barrier tissues and studied the possibility of using impedance spectroscopy to solve this problem. It was demonstrated (theoretical...
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Article
The Biochemical Model of the Synapse in Turpaev’s Studies
The concept of specific receptors is the basis of the modern pharmacology of neurotransmitters. This article describes the works of Tigran Mel’kumovich Turpaev (1918–2003), during which a receptor protein was ...
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Article
Elimination of a Viscumin-Ferromagnetic Nanoparticles Conjugate from the Tumor Nodule in Mice
External magnetic field is characterized by low toxicity and existence of magnetic properties, which contributes to an interest in the development of products from ferromagnetic nanoparticles (FNP) for antitum...
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Article
Target Cell Glycosylation Determines the Biodistribution of Plant Lectin Viscumin
We studied the possibility of using viscumin lectin (MLI) for targeted delivery of antitumor drugs. Affinity of MLI for more than 600 oligosaccharide structures was determined and the glycosylation profiles of...
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Article
Development of a Specific Substrate—Inhibitor Panel (Liver-on-a-Chip) for Evaluation of Cytochrome P450 Activity
We developed a cytochrome P450 substrate—inhibitor panel for preclinical in vitro evaluation of drugs in a 3D histotypical microfluidic cell model of human liver (liver-on-a-chip technology). The concentration...
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Article
Effect of Circulation Parameters on Functional Status of HepaRG Spheroids Cultured in Microbioreactor
We studied the relationship between microcirculation parameters and functional status of HepaRG cells in spheroids and chose an optimal regimen within the physiologically permissible limits of mechanical impac...
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Article
Modeling of Magnetite Nanoparticles Behavior under Conditions of Microcirculation and Analysis of In Vivo Toxicity
The behavior of magnetite nanoparticles was studied in the cell chip microcapillaries. No aggregation of magnetite nanoparticles under conditions of long-term circulation was noted. Biodistribution and toxicit...
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Article
Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium
We studied the effects of DMSO and fibroblasts during HepaRG cell spheroid formation and conditions of their subsequent culturing on the levels of mRNA of the major cytochromes P450. A protocol of spheroid for...
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Article
Complex Approach to Xenobiotics Hepatotoxicity Testing using a Microfluidic System
We analyzed hepatotoxicity of three drugs: acetaminophen, metformin, and isoniazid. Spheroids of differentiated HepaRG cells cultured under microfluidic conditions were used as the model. Acute toxicity of sub...
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Article
p53- and Caspase-3-Independent Mechanism of Acetaminophen Effect on Human Neural Cells
Acetaminophen in a concentration of 5 mM increased the expression of JNK, HIF1A (hypoxiainduced factor), and CASP3, which indicated development of oxidative stress and apoptotic cell death. Acetaminophen in a con...
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Article
Preparation of Viscumin-Ferromagnetic Particles Conjugate and Study of Its Internalization by Human Glioblastoma A172 Cells
Magnetite particles modified by polyethylene glycol with a molecular weight of 3 kDa and hydrodynamic diameter of ~60 nm were used. Plant lectin viscumin covalently immobilized on these nanoparticles retained ...
