Magnetite particles modified by polyethylene glycol with a molecular weight of 3 kDa and hydrodynamic diameter of ~60 nm were used. Plant lectin viscumin covalently immobilized on these nanoparticles retained its binding activity. Immunochemical characteristics of conjugated viscumin were evaluated using monoclonal antibodies. The resultant conjugate with a hydrodynamic diameter of 70 nm was used for studies of binding and internalization by target cells. Binding of viscumin and its conjugate was determined by receptors containing terminal galactose, while intracellular distribution varied. The model system presented in this study can be used for creation of drugs for target therapy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
I. I. Agapov, A. G. Tonevitsky, N. V. Maluchenko, et al., FEBS Lett., 464, Nos. 1-2, 63-66 (1999).
I. I. Agapov, A. G. Tonevitsky, M. M. Moysenovich, et al., FEBS Lett., 452, No. 3, 211-214 (1999).
I. I. Agapov, A. G. Tonevitsky, A. T. Shamsiev, et al., FEBS Lett., 402, No. 1, 91-93 (1997).
M. Bloemen, T. Van Stappen, P. Willot, et al., PLoS One, 9, No. 10, doi: 10.1371/journal.pone.00109475 (2014).
O. L. Gobbo, K. Sjaastad, M. W. Radomski, et al., Theranostics, 5, No. 11, 1249-1263 (2015).
T. G. Iversen, N. Frerker, and K. Sandvig, J. Nanobiotechnology, 10, doi: 10.1186/1477-3155-10-33 (2012).
M. M. Moisenovich, A. G. Tonevitsky, N. V. Maljuchenko, et al., Eur. J. Cell. Biol., 81, No. 10, 529-538 (2002).
M. M. Moisenovich, A. G. Tonevitsky, N. V. Maljuchenko, et al., Histochem. Cell Biol., 121, No. 6, 429-439 (2004).
H. Niwa, A. G. Tonevitsky, I. I. Agapov, et al., Eur. J. Biochem., 270, No. 13, 2739-2749 (2003).
P. Pohl, Y. N. Antonenko, V. Y. Evtodienko, et al., Biochim. Biophys. Acta, 1371, No. 1, 11-16 (1998).
N. V. Pulkova, S. A. Tonevitskaya, V. M. Gerasimov, et al., Nanotechnologies in Russia, 10, Nos. 7-8 (2015).
B. Saha, T. H. Evers, and M. W. Prins, Anal. Chem., 86, No. 16, 8158-8166 (2014).
E. C. Sweeney, A. G. Tonevitsky, R. A. Palmer, et al., FEBS Lett., 431, No. 3, 367-370 (1998).
A. G. Tonevitsky, I. I. Agapov, A. T. Shamsiev, et al., FEBS Lett., 392, No. 2, 166-168 (1996).
A. G. Tonevitsky, I. I. Agapov, D. Temiakov, et al., Arzneimittelforschung, 49, No. 11, 970-975 (1999).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 160, No. 12, pp. 797-801, December, 2015
Rights and permissions
About this article
Cite this article
Khutornenko, A.A., Gerasimov, V.M. & Sakharov, D.A. Preparation of Viscumin-Ferromagnetic Particles Conjugate and Study of Its Internalization by Human Glioblastoma A172 Cells. Bull Exp Biol Med 160, 823–826 (2016). https://doi.org/10.1007/s10517-016-3319-0
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-016-3319-0