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Article
Modeling the Distribution of the Electromagnetic Field inside a Microcuvette Designed for Measuring the Impedance Spectra of Three-Dimensional Cell Cultures
Spectra obtained using a four-electrode measurement scheme in a medium with dispersion are modeled mathematically using the example of a three-dimensional cell culture. The problem is solved with the finite el...
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Article
Develo** a Microfluidic Device with an Integrated Electrode System for Measuring the Impedance Spectra of Cellular Models in Real Time
A mathematical model is presented that describes the distribution of the potential and electric field strength in cells of a microfluidic device for culturing a cellular monolayer with built-in electrodes of d...
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Article
Transport and toxicity of 5-fluorouracil, doxorubicin, and cyclophosphamide in in vitro placental barrier model based on BeWo b30 cells
An in vitro placental barrier model based on human choriocarcinoma BeWo b30 cell line was considered as a method of preclinical study of the transport and toxicity of antitumor agents and other organic compounds....
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Article
Impedance Spectroscopy and Transcriptome Analysis of Choriocarcinoma BeWo b30 as a Model of Human Placenta
Placenta is a highly specialized organ that is necessary for successful gestation. Several models of the placental barrier are used to study how it functions, including the transplacental transport of xenobiot...
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Article
Comparison of Profiles of Extracellular MicroRNA Secreted by Caco-2 Cells from the Apical Side of the Membrane under Static and Microcirculation Conditions
Extracellular microRNA are one of the indicators of the functional state of cells. Culturing of Caco-2 cells under the conditions of microcirculation in a Homunculus microfluidic device allows better simulatin...
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Article
Application of Impedance Spectroscopy for the Control of the Integrity of In Vitro Models of Barrier Tissues
We compared available methods for monitoring the integrity of in vitro models of barrier tissues and studied the possibility of using impedance spectroscopy to solve this problem. It was demonstrated (theoretical...
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Article
Oxyquinoline-Dependent Changes in Claudin-Encoding Genes Contribute to Impairment of the Barrier Function of the Trophoblast Monolayer
Natural response to hypoxia critically depends on rapid stabilization of hypoxia-inducible factor (HIF). Under normoxic conditions, HIF-prolyl hydroxylases mark α-subunits of HIF for degradation, while hypoxia...
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Article
Non-Invasive Evaluation of Extracellular Matrix Formation in the Intestinal Epithelium
Differentiation of colorectal cancer Caco-2 cells was assessed using Affymetrix Human Gene 1.0 ST arrays and by the main electrical parameters measured by bioimpedance spectroscopy. Transepithelial electrical ...
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Article
Expression of SLC30A10 and SLC23A3 Transporter mRNAs in Caco-2 Cells Correlates with an Increase in the Area of the Apical Membrane
Drug bioavailability studies commonly employ in vitro barrier tissue models consisting of epithelial and endothelial cells. These experiments require that the cell barrier quality be assessed regularly, which ...
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Article
Features of the Temperature Distribution in Flat Conductors with Different Configurations
Steady-state temperature distributions in flat conductors of different configurations with direct electric current flowing inside them are investigated. It is shown that the temperature distributions are deter...
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Article
Experimental investigation of a magnetic field near strip conductors with rectangular cutouts
A theoretical distribution of the magnetic fields near rectangular cutouts in strip conductors of different widths is derived. An experimental investigation of the magnetic fields of the strip conductors is pe...
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Article
Effect of Circulation Parameters on Functional Status of HepaRG Spheroids Cultured in Microbioreactor
We studied the relationship between microcirculation parameters and functional status of HepaRG cells in spheroids and chose an optimal regimen within the physiologically permissible limits of mechanical impac...
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Article
A Model of Cadmium Uptake and Transport in Caco-2 Cells
We created a physiologically substantiated kinetic model of cadmium transport and toxicity in intestinal cell model (Caco-2 cells). Transcriptome profiling of Caco-2 cells revealed high content of transporter ...
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Article
Localization and Expression of Nucleoside Transporters ENT1 and ENT2 in Polar Cells of Intestinal Epithelium
Сасо-2 cell line is widely used in in vitro studies of the intestinal wall permeability for drugs, but transport activity of these cells has not been thoroughly studied. We analyzed localization and expression of...
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Article
Mathematical and Experimental Model of Oxygen Diffusion for HepaRG Cell Spheroids
3D cell cultures are extensively used to study in vitro toxic effect of xenobiotics. When using multicellular spheroids, the question about their optimal size should be solved: small spheroids are difficult to ma...
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Article
Transport and Toxicity of Silver Nanoparticles in HepaRG Cell Spheroids
We studied the effects of silver nanoparticles (10 nm) on HepaRG cell spheroids simulating liver tissue. The mathematical model was proposed that describes nanoparticle diffusion in a spheroid consisting of 50...
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Article
Control over electronic components by their magnetic field
We show the results of our studies related to control over objects by the magnetic field they generate. We propose a model to compute the current and its magnetic field in a conductor with a rectangular defect...
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Article
Conformal transformation method as applied to finding the current density distribution and induced magnetic field in a strip conductor with a rectangular cut
Given a conductor with a rectangular cut, the problem of determining the direct current distribution and the induced magnetic field in the conductor is solved with applications to the testing of chip conductor...
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Article
A gradient magnetic field for control of magnetic micro- and nanobeads in a viscous medium
This paper presents the results of theoretical analysis of motion control for magnetic micro- and nanobeads in a gradient magnetic field in a viscous medium. The problem considered is relevant for the design o...
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Article
The temperature distribution in a flat conductive disk heated by a direct current
The problem of the steady-state temperature distribution in a flat conductive disk that is heated by a direct current was investigated. An analytical solution was obtained and limiting cases corresponding to d...
