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  1. Article

    Open Access

    The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - a yeast of biotechnological interest

    The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a ...

    Gotthard Kunze, Claude Gaillardin, Małgorzata Czernicka in Biotechnology for Biofuels (2014)

  2. No Access

    Article

    An overview of lipid metabolism in yeasts and its impact on biotechnological processes

    High energy prices, depletion of crude oil supplies, and price imbalance created by the increasing demand of plant oils or animal fat for biodiesel and specific lipid derivatives such as lubricants, adhesives,...

    Athanasios Beopoulos, Jean-Marc Nicaud in Applied Microbiology and Biotechnology (2011)

  3. No Access

    Chapter

    Applications of the Non-Conventional Yeast Yarrowia lipolytica

    The yeast Yarrowia lipolytica is often found associated to proteinaceous or hydrophobic substrates such as alkanes or lipids. To assimilate these hydropho-bic substrates, Y. lipolytica has developed an adaptative...

    France Thevenieau, Jean-Marc Nicaud in Yeast Biotechnology: Diversity and Applica… (2009)

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    Article

    Functional Analysis of the MATB Mating-Type Idiomorph of the Dimorphic Fungus Yarrowia lipolytica

    The whole MATA cassette from Yarrowia lipolytica, a dimorphic fungus, was replaced by the URA3 gene through a double homologous recombination. This MAT-less strain lost its mate capacity with A or B Y. lipolytica

    Raymundo Rosas-Quijano, Claude Gaillardin, José Ruiz-Herrera in Current Microbiology (2008)

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    Article

    Chromosomal polymorphism in the yeast species Debaryomyces hansenii

    Pulse field gel electrophoresis karyotypes of 41 strains of the genus Debaryomyces, including 35 strains confirmed as D. hansenii species by D1/D2 ribosomal DNA sequence analysis, were performed. Electrophoretic ...

    Mauricio Corredor, Anne-Marie Davila, Serge Casarégola in Antonie van Leeuwenhoek (2003)

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    Article

    Chromosomal polymorphism in the yeast species Debaryomyces hansenii

    Pulse field gel electrophoresis karyotypes of 41 strains of the genus Debaryomyces, including 35 strains confirmed as D. hansenii species by D1/D2 ribosomal DNA sequence analysis, were performed. Electrophoretic ...

    Mauricio Corredor, Anne-Marie Davila, Serge Casarégola in Antonie van Leeuwenhoek (2003)

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    Article

    Cloning the Yarrowia lipolytica homologue of the Saccharomyces cerevisiae SEC62 gene

    Yarrowia lipolytica SEC62 cDNA was cloned by functional complementation of a thermo-sensitive sec62 Saccharomyces cerevisiae mutant strain. The Y. lipolytica SEC62 promoter region was amplified by the inverse po...

    D. Swennen, Philippe Joyet, Claude Gaillardin in Current Genetics (1997)

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    Chapter

    Yarrowia lipolytica

    Interest in Candida lipolytica (Harrison) Diddens et Lodder 1942 initially arose from its rather uncommon physiological characteristics. Strains of this species were more often isolated from lipid- or protein-con...

    Gerold Barth, Claude Gaillardin in Nonconventional Yeasts in Biotechnology (1996)

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    Article

    Molecular cloning of Rab-related genes in the yeast Yarrowia lipolytica. Analysis of RYL1, an essential gene encoding a SEC4 homologue

    Small GTP-binding proteins of the Rab family are involved in the vesicular traffic inside eukaryotic cells. A gene library from the yeast Yarrowia lipolytica was screened with an oligonucleotide deduced from a hi...

    Brigitte Pertuiset, Jean-Marie Beckerich, Claude Gaillardin in Current Genetics (1995)

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    Article

    Multiple-copy integration in the yeast Yarrowia lipolytica

    Using an EcoRI-BglII fragment of the G unit of the rDNA of Y. lipolytica and a set of 11 deletions in the URA3 promoter, we have constructed several plasmids to test gene amplification in the rDNA. These plasmids...

    Marie-Thérèse Le Dall, Jean-Marc Nicaud, Claude Gaillardin in Current Genetics (1994)

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    Article

    LYC1 is the structural gene for lysine N-6-acetyl transferase in yeast

    In the yeastYarrowia lipolytica, theLYC1 locus controls the first step of the lysine degradation pathway which is catalyzed by lysine N-6-acetyl transferase (LAT). This gene was cloned by complementation of thely...

    Jean-Marie Beckerich, Micheline Lambert, Claude Gaillardin in Current Genetics (1994)

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    Article

    Chromosomal polymorphism of the yeast Yarrowia lipolytica and related species: electrophoretic karyoty** and hybridization with cloned genes

    Significant differences in electrophoretic karyoty** patterns were found among 27 strains of Y. lipolytica. Twenty-one of these strains were classified into four groups of similar karyotypes while six strains s...

    Elena Naumova, Gennadi Naumov, Philippe Fournier, Huu-Vang Nguyen in Current Genetics (1993)

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    Article

    The yeast Yarrowia lipolytica has two, functional, signal recognition particle 7S RNA genes

    Cells containing a deletion of either the SCR1 or SCR2 genes, which code for the 7SL RNA component of the signal recognition particle (SRP) homologue, were found to be viable. Two independent approaches demonstra...

    Feng He, Debbie Yaver, Jean-Marie Beckerich, David Ogrydziak in Current Genetics (1990)

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    Article

    Expression of invertase activity in Yarrowia lipolytica and its use as a selective marker

    Few selective markers are available for the transformation of the industrial yeast Yarrowia lipolytica, and those that are require the use of specialized hosts (e.g., auxotrophs, antibiotic sensitive). To enable ...

    Jean-Marc Nicaud, Emmanuelle Fabre, Claude Gaillardin in Current Genetics (1989)

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    Article

    Cloning of the LYS5 gene encoding saccharopine dehydrogenase from the yeast Yarrowia lipolytica by target integration

    A Yarrowia lipolytica yeast gene bank has been constructed in E. coli in an integrative plasmid vector containing the homologous LEU2 gene, and used to transform a leu2 lys5 yeast strain. The LYS5 gene encoding s...

    Jian-Wu Xuan, Philippe Fournier, Claude Gaillardin in Current Genetics (1988)

  16. No Access

    Article

    LEU2 directed expression of β-galactosidase activity and phleomycin resistance in Yarrowia lipolytica

    The nucleotide sequence of a 968 by DNA fragment spanning the promoter and the 5′ upstream sequence of the LEU2 coding sequence of the yeast Yarrowia lipolytica has been determined. A LEU2:lacZ gene fusion has be...

    Claude Gaillardin, Anne-Marie Ribet in Current Genetics (1987)

  17. No Access

    Article

    Replication and recombination of 2-µm DNA in Schizosaccharomyces pombe

    Any one of the inverted sequences present on the 2-µm DNA from Saccharomyces cerevisiae can promote replication of chimeric plasmids in Schizosaccharomyces pombe. When however a complete 2-µm molecule is present ...

    Claude Gaillardin, Philippe Fournier, Françoise Budar, Bernard Kudla in Current Genetics (1983)