Translational Cardiology
Molecular Basis of Cardiac Metabolism, Cardiac Remodeling, Translational Therapies and Imaging Techniques
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Article
Genomic structure of the human KDR/flk-1 gene
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Chapter
Angiotensin II induces gene transcription through cell-type-dependent effects on the nuclear factor-кB (NF-кB) transcription factor
The vasopressor octapeptide, angiotensin II (Ang II), exerts homeostatic responses in cardiovascular tissues, including the heart, blood vessel wall, adrenal cortex and liver (a major source of circulating pla...
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Article
Angiotensin II induces gene transcription through cell-type-dependent effects on the nuclear factor-κB (NF-κB) transcription factor
The vasopressor octapeptide, angiotensin II (Ang II), exerts homeostatic responses in cardiovascular tissues, including the heart, blood vessel wall, adrenal cortex and liver (a major source of circulating pla...
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Article
The co-chaperone CHIP regulates protein triage decisions mediated by heat-shock proteins
To maintain quality control in cells, mechanisms distinguish among improperly folded peptides, mature and functional proteins, and proteins to be targeted for degradation. The molecular chaperones, including h...
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Article
The Hsc70 co-chaperone CHIP targets immature CFTR for proteasomal degradation
The folding of both wild-type and mutant forms of the cystic-fibrosis transmembrane-conductance regulator (CFTR), a plasma-membrane chloride-ion channel, is inefficient1,2,3,4. Most nascent CFTR is retained in th...
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Article
Shear Stress Reduces Protease Activated Receptor-1 Expression in Human Endothelial Cells
Shear stress has been shown to regulate several genes involved in the thrombotic and proliferative functions of endothelial cells. Thrombin receptor (protease-activated receptor-1: PAR-1) increases at sites of...
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Protocol
Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells
Components of the ubiquitin-proteasome system function on the surface of the endoplasmic reticulum (ER) to select misfolded proteins for degradation. Herein we describe methods that allow for the study of the ...
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Article
CHIP-mediated stress recovery by sequential ubiquitination of substrates and Hsp70
CHIP — a co-chaperone/ubiquitin ligase — not only targets chaperone substrates for degradation, but mediates Hsp70 turnover after misfolded substrates have been depleted. The sequential catalysis of the CHIP-a...
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Chapter
Up and Down Regulation of the Stress Response by the Co-Chaperone Ubiquitin Ligase CHIP
Following exposure to environmental insults, the cells in most tissues dramatically increase the production of a group of proteins that are collectively known as “heat shock” or stress proteins (Parsell and Lindq...
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Article
Therapeutic options for premature coronary artery disease
Although cardiovascular disease is commonly recognized as a disease of the elderly, young patients are also at risk for coronary atherosclerosis, which has a devastating impact on their more active lifestyle. ...
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Article
Open AccessTobacco use induces anti-apoptotic, proliferative patterns of gene expression in circulating leukocytes of Caucasian males
Strong epidemiologic evidence correlates tobacco use with a variety of serious adverse health effects, but the biological mechanisms that produce these effects remain elusive.
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Article
Structural basis of nucleotide exchange and client binding by the Hsp70 cochaperone Bag2
Bag2 acts as a nucleotide-exchange factor for Hsp70 and also binds misfolded substrates. Now structural work reveals that Bag2 promotes nucleotide exchange via a mechanism distinct from other Hsp70 nucleotide-...
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Chapter
The Role of Thrombin in Vascular Development
Vasculogenesis is essential for embryonic development. The vasculature and the intravascular blood compartment develop in a close spatial and temporal relationship. Here we discuss how thrombin, as the common ...
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Article
Torturing a blood vessel
Experiments in mice and zebrafish uncover a pathway behind malformed blood vessels in the brain (pages 169–176 & 177–184
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Article
Breast cancer quality control
Tumorigenesis is regulated by several mechanisms including signalling, transcription and DNA replication. Now a cytoplasmic protein quality-control pathway is implicated in the suppression of breast cancer cel...
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Article
A Good Idea: A Physician’s Perspective on Genetic Counseling for Hypertrophic Cardiomyopathy
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Article
Seek and destroy: The ubiquitin-proteasome system in cardiac disease
The ubiquitin-proteasome system (UPS) is a major proteolytic system that regulates the degradation of intracellular proteins in the heart. The UPS regulates the turnover of misfolded and damaged proteins, in a...
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Protocol
Reconstitution of CHIP E3 Ubiquitin Ligase Activity
CHIP, the carboxyl-terminus of Hsp70 interacting protein, is both an E3 ubiquitin ligase and an Hsp70 co-chaperone and is implicated in the degradation of cytosolic quality control and numerous disease substra...
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Open AccessAnkyrin repeat and SOCS box containing protein 4 (Asb-4) colocalizes with insulin receptor substrate 4 (IRS4) in the hypothalamic neurons and mediates IRS4 degradation
The arcuate nucleus of the hypothalamus regulates food intake. Ankyrin repeat and SOCS box containing protein 4 (Asb-4) is expressed in neuropeptide Y and proopiomelanocortin (POMC) neurons in the arcuate nucl...
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