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Open AccessASAP: Amplification, sequencing & annotation of plastomes
Availability of DNA sequence information is vital for pursuing structural, functional and comparative genomics studies in plastids. Traditionally, the first step in mining the valuable information within a chl...
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Article
Open AccessRapid and accurate pyrosequencing of angiosperm plastid genomes
Plastid genome sequence information is vital to several disciplines in plant biology, including phylogenetics and molecular biology. The past five years have witnessed a dramatic increase in the number of comp...
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Open AccessComparative chloroplast genomics and phylogenetics of Fagopyrum esculentum ssp. ancestrale– A wild ancestor of cultivated buckwheat
Chloroplast genome sequences are extremely informative about species-interrelationships owing to its non-meiotic and often uniparental inheritance over generations. The subject of our study, Fagopyrum esculentum,...
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Open AccessWhole genome sequencing of peach (Prunus persica L.) for SNP identification and selection
The application of next generation sequencing technologies and bioinformatic scripts to identify high frequency SNPs distributed throughout the peach genome is described. Three peach genomes were sequenced usi...
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Article
Open AccessRapid gene-based SNP and haplotype marker development in non-model eukaryotes using 3'UTR sequencing
Sweet cherry (Prunus avium L.), a non-model crop with narrow genetic diversity, is an important member of sub-family Amygdoloideae within Rosaceae. Compared to other important members like peach and apple, sweet ...
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Open AccessDevelopment of a highly efficient Axiom™ 70 K SNP array for Pyrus and evaluation for high-density map** and germplasm characterization
Both a source of diversity and the development of genomic tools, such as reference genomes and molecular markers, are equally important to enable faster progress in plant breeding. Pear (Pyrus spp.) lags far behi...
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Open AccessGlyoxylic acid overcomes 1-MCP-induced blockage of fruit ripening in Pyrus communis L. var. ‘D’Anjou’
1-methylcyclopropene (1-MCP) in an ethylene receptor antagonist that blocks ethylene perception and downstream ripening responses in climacteric fruit imparting a longer shelf life. However, in European pear, ...
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Article
Open AccessGenome-Scale Characterization of Predicted Plastid-Targeted Proteomes in Higher Plants
Plastids are morphologically and functionally diverse organelles that are dependent on nuclear-encoded, plastid-targeted proteins for all biochemical and regulatory functions. However, how plastid proteomes va...
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Open AccessEvidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit
European pear (Pyrus communis L.) cultivars require a genetically pre-determined duration of cold-temperature exposure to induce autocatalytic system 2 ethylene biosynthesis and subsequent fruit ripening. The phy...
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Article
Open AccessMethods of analysis of chloroplast genomes of C3, Kranz type C4 and Single Cell C4 photosynthetic members of Chenopodiaceae
Chloroplast genome information is critical to understanding forms of photosynthesis in the plant kingdom. During the evolutionary process, plants have developed different photosynthetic strategies that are acc...
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Preface: Genome editing in plants
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Open AccessImpact of heat stress, water stress, and their combined effects on the metabolism and transcriptome of grape berries
Recurring heat and drought episodes present challenges to the sustainability of grape production worldwide. We investigated the impacts of heat and drought stress on transcriptomic and metabolic responses of b...