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Open AccessA Case Study from the Overexpression of OsTZF5, Encoding a CCCH Tandem Zinc Finger Protein, in Rice Plants Across Nineteen Yield Trials
Development of transgenic rice overexpressing transcription factors involved in drought response has been previously reported to confer drought tolerance and therefore represents a means of crop improvement. W...
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Article
Open AccessProof of concept and early development stage of market-oriented high iron and zinc rice expressing dicot ferritin and rice nicotianamine synthase genes
Micronutrient deficiencies such as iron (Fe), zinc (Zn), and vitamin A, constitute a severe global public health phenomenon. Over half of preschool children and two-thirds of nonpregnant women of reproductive ...
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Article
Genome-edited crops for improved food security of smallholder farmers
Widespread enthusiasm about potential contributions of genome-edited crops to address climate change, food security, nutrition and health, environmental sustainability and diversification of agriculture is dam...
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Preface: Genome editing in plants
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Article
Open AccessGenome editing in cereal crops: an overview
Genome-editing technologies offer unprecedented opportunities for crop improvement with superior precision and speed. This review presents an analysis of the current state of genome editing in the major cereal...
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Article
Genome editing reagent delivery in plants
Genome editing holds the potential for rapid crop improvement to meet the challenge of feeding the planet in a changing climate. The delivery of gene editing reagents into the plant cells has been dominated by...
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Protocol
Rice Gene Knockout or Downregulation through CRISPR-Cas9
Precise genome modification via clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas)9 in rice plants, is a powerful tool for improving abiotic and biotic stress to...
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Article
Open AccessBroad-spectrum resistance to bacterial blight in rice using genome editing
Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind s...
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Chapter
Biofortified Zn and Fe Rice: Potential Contribution for Dietary Mineral and Human Health
Iron (Fe) and zinc (Zn) deficiency constitute a major micronutrient deficiency around the globe, affecting rural populations residing in develo** countries with minimum purchasing power and/or access to a di...
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Article
Open AccessAdvances in breeding for high grain Zinc in Rice
Zinc (Zn) is one of the most essential micronutrients required for the growth and development of human beings. More than one billion people, particularly children and pregnant women suffer from Zn deficiency r...
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Article
Open AccessBiofortified indica rice attains iron and zinc nutrition dietary targets in the field
More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g−1 iron (Fe) and 16 μg g−1 zinc (Zn). The HarvestPlus breeding program...
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Article
A trehalose-6-phosphate phosphatase enhances anaerobic germination tolerance in rice
Global socioeconomic developments create strong incentives for farmers to shift from transplanted to direct-seeded rice (DSR) as a means of intensification and economization1. Rice production must increase to ens...
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Protocol
Agrobacterium-Mediated Transformation: Rice Transformation
Agrobacterium is a common soil bacterium with natural capacity for trans-kingdom transfer of genetic information by transferring its T-DNA into the eukaryotic genome. In agricultural plant biotechn...
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Article
Open AccessLarge-scale production and evaluation of marker-free indica rice IR64 expressing phytoferritin genes
Biofortification of rice (Oryza sativa L.) using a transgenic approach to increase the amount of iron in the grain is proposed as a low-cost, reliable, and sustainable solution to help develo** countries combat...
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Article
Open AccessEvolution of GOLDEN2-LIKE gene function in C3 and C4 plants
A pair of GOLDEN2-LIKE transcription factors is required for normal chloroplast development in land plant species that encompass the range from bryophytes to angiosperms. In the C4 plant maize, compartmentalized ...
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Article
A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members
The homeodomain leucine zipper (HD-Zip) genes encode transcription factors that have diverse functions in plant development and have often been implicated in stress adaptation. The HD-Zip genes are the most ab...