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Article
Open AccessAmplification sensing manipulated by a sumanene-based supramolecular polymer as a dynamic allosteric effector
The synthesis of signal-amplifying chemosensors induced by various triggers is a major challenge for multidisciplinary sciences. In this study, a signal-amplification system that was flexibly manipulated by a ...
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Article
Diffusion behavior of rhodamine 6G in single octadecylsilyl-functionalized silica particle revealed by fluorescence correlation spectroscopy
We investigated the diffusion behavior of rhodamine 6G (Rh6G) within single octadecylsilyl-functionalized (ODS) silica particle in an acetonitrile (ACN)/water system using fluorescence correlation spectroscopy...
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Article
Evolution of myoglobin diffusion mechanisms: exploring pore and surface diffusion in a single silica particle
This study elucidates the mass transfer mechanism of myoglobin (Mb) within a single silica particle with a 50 nm pore size at various pH levels (6.0, 6.5, 6.8, and 7.0). Investigation of Mb distribution ratio (R)...
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Article
Kinetic analysis of mass transfer of Eu(III) in extractant-impregnated polymer-layered silica particle in multiple-ion distribution system
The Eu(III) distribution mechanism in single extractant-impregnated polymer-layered silica particle in a complex solution containing multiple lanthanide ions was investigated using fluorescence microspectrosco...
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Article
Investigating hydrophobic environment in alkyl-group-functionalized silica particle with various chain lengths using absorption microspectroscopy
A well-known solvatochromic dye, Reichardt’s dye (R-dye), was used to evaluate the hydrophobicity of alkyl-group-functionalized silica particles (ASPs) with different chain lengths. The absorption spectra of R...
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Article
Eu(III) transfer in single N,N,N’,N’-tetraoctyldiglycolamide-impregnated polymer-coated silica particle using fluorescence microspectroscopy: transfer mechanism and effect of polymer crosslinking degree
A microcapillary manipulation system combined with fluorescence microspectroscopy enabled us to analyze mass transfer in a single particle. In this study, we revealed the Eu(III) distribution in a single digly...
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Article
J-aggregation of 5, 10, 15, 20-tetraphenyl-21H, 23H-porphinetetrasulfonic acid in a molecular crowding environment simulated using dextran
In a molecular crowding environment, different thermodynamics is often observed in a dilute solution. One such example is the promotion of the formation of amyloids, which are causal agents of Alzheimer’s dise...
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Article
Kinetics and mechanism of Eu(III) transfer in tributyl phosphate microdroplet/HNO3 aqueous solution system revealed by fluorescence microspectroscopy
In this study, we reveal an Eu(III) extraction mechanism at the interface between HNO3 and tributyl phosphate (TBP) solutions using fluorescence microspectroscopy. The mass transfer rate constant at the interface...
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Chapter
Principle of Detection Based on Particle Levitation in Coupled Acoustic-Gravitational Field
In this chapter, the detection principle based on particle levitation in a coupled acoustic-gravitational (CAG) field is theoretically described. A detection concept based on particle levitation is described i...
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Chapter
Label-Free Detection for DNA/RNA Molecules
In Chapter 4, a zmol-sensing scheme based on the levitation behavior of a single microparticle is proposed. However, this detection scheme needs the anchoring of target ...
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Chapter
Conclusion and Outlook
Acoustic levitation-based biosensing at the zmol level was demonstrated in this thesis. This method is versatile and can be tailored to detect a variety of targets, such as proteins, coenzymes, nucleotides, an...
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Chapter
Theory of Combined Acoustic-Gravitational Field
In this chapter, the principle of an acoustic radiation force and a combined acoustic-gravitational (CAG) field is discussed. From Sect. 2.1 to Sect. 2.4, the mathematical interpretation of the acoustic radiation...
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Chapter
Detection of the Avidin–Biotin Reaction
In this section, a detection scheme based on the levitation coordinate shift of a microparticle by the binding of AuNPs is demonstrated. The relationship between the particle density and the levitation coordin...
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Chapter
Aptamer-Based Sensing of Small Organic Molecules
In this chapter, a system based on sandwich hybridization is applied to the detection of small organic molecules using an aptamer. Aptamer is a DNA molecule with a specific sequence that selectively binds to a...
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Chapter
Introduction
In this chapter, the background of ultrasound and the application of particle separation using various external fields are introduced. The features and applications of ultrasound are discussed in Sect. 1.1. Parti...
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Article
Particle Manipulation with External Field; From Recent Advancement to Perspectives
Physical forces, such as dielectric, magnetic, electric, optical, and acoustic force, provide useful principles for the manipulation of particles, which are impossible or difficult with other approaches. Micro...
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Article
Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles
Herein, we propose a concept for sensing based on density changes of microparticles (MPs) caused by a biochemical reaction. The MPs are levitated by a combined acoustic-gravitational force at a position determ...
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Article
Comparative studies on the fatty acid composition of moderately and extremely thermophilic bacteria
The fatty acids of three strains of extremely thermophilic bacteria and three strains of moderately thermophilic bacteria were examined by gas liquid chromatography. All the thermophiles contained straight, is...
