Abstract
In Chapter 4, a zmol-sensing scheme based on the levitation behavior of a single microparticle is proposed. However, this detection scheme needs the anchoring of target molecules on the microparticle or AuNP, which may lead to a change in the chemical and/or physical properties of the target molecules. In this chapter, a label-free detection scheme using DNA/RNA sandwich hybridization is demonstrated. In Sect. 5.2, the effect of the bp number, involved in two direct complementary hybridizations, on sensitivity is investigated before the sandwich hybridization scheme is examined. Based on the knowledge obtained in Sect. 5.2, detection schemes are designed to quantify the HIV-2 DNA and single nucleotide polymorphism by sandwich hybridization in Sect. 5.3. This approach is applied to the quantification of multiple microRNA (miRNA) molecules in Sect. 5.4.
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Miyagawa, A. (2021). Label-Free Detection for DNA/RNA Molecules. In: Acoustic Levitation-Based Trace-Level Biosensing. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-16-1425-5_5
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DOI: https://doi.org/10.1007/978-981-16-1425-5_5
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