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  1. No Access

    Article

    Gibberellin A3 as an epigenetic determinant of global DNA hypo-methylation in tobacco

    Gibberellins (GAs) are a large family of tetracyclic diterpenoids, controlling important aspects of growth and development throughout the plant life cycle. To explore the possibility that gibberellin A3 (GA3) sig...

    R. Manoharlal, G. V. S. Saiprasad, C. Ullagaddi, A. Kovařík in Biologia Plantarum (2018)

  2. No Access

    Article

    Concerted evolution rapidly eliminates sequence variation in rDNA coding regions but not in intergenic spacers in Nicotiana tabacum allotetraploid

    Nicotiana tabacum (tobacco) is a natural allotetraploid that formed from two diploid progenitors (N. sylvestris—S-genome, N. tomentosiformis—T-genome) within past 0.2 million years. Previ...

    J. Lunerová, S. Renny-Byfield, R. Matyášek, A. Leitch… in Plant Systematics and Evolution (2017)

  3. Article

    Silenced rRNA genes are activated and substitute for partially eliminated active homeologs in the recently formed allotetraploid, Tragopogon mirus (Asteraceae)

    To study the relationship between uniparental rDNA (encoding 18S, 5.8S and 26S ribosomal RNA) silencing (nucleolar dominance) and rRNA gene dosage, we studied a recently emerged (within the last 80 years) allo...

    E Dobešová, H Malinská, R Matyášek, A R Leitch, D E Soltis, P S Soltis… in Heredity (2015)

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  4. Article

    Open Access

    Dancing together and separate again: gymnosperms exhibit frequent changes of fundamental 5S and 35S rRNA gene (rDNA) organisation

    In higher eukaryotes, the 5S rRNA genes occur in tandem units and are arranged either separately (S-type arrangement) or linked to other repeated genes, in most cases to rDNA locus encoding 18S–5.8S–26S genes ...

    S Garcia, A Kovařík in Heredity (2013)

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  5. Article

    Frequent silencing of rDNA loci on the univalent-forming genomes contrasts with their stable expression on the bivalent-forming genomes in polyploid dogroses (Rosa sect. Caninae)

    The polyploid species in Rosa section Caninae (2n=21, 28 or 35) are characterized by an unusual reproductive system known as odd (or asymmetric) meiosis. Only two chromosome sets form bivalents in meiosis, wherea...

    L Khaitová, G Werlemark, H Nybom, A Kovařík in Heredity (2010)

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  6. No Access

    Article

    Distribution of 5-methylcytosine residues in 5S rRNA genes in Arabidopsis thaliana and Secale cereale

    Bisulfite genomic sequencing was used to localise 5-methylcytosine residues (mC) in 5S rRNA genes of Arabidopsis thaliana and Secale cereale. The maps of mC distribution were compared with the previously publish...

    J. Fulneček, R. Matyášek, A. Kovařík in Molecular Genetics and Genomics (2002)

  7. No Access

    Article

    Transposition of IS10 from the host Escherichia coli genome to a plasmid may lead to cloning artefacts

    During recloning of Nicotiana tabacum L. repetitive sequence R8.3 in Escherichia coli, a modified clone that differed from the original by the insertion of an IS10 sequence was unintentionally produced. The inse...

    A. Kovařík, M. Matzke, A. Matzke, B. Koukalová in Molecular Genetics and Genomics (2001)

  8. No Access

    Article

    Comparative analysis of DNA methylation in tobacco heterochromatic sequences

    Cytosine methylation levels and susceptibility to drug-induced hypomethylation have been studied in several Nicotiana tabacum (tobacco) DNA repetitive sequences. It has been shown using HapII, MspI, BamHI and Sau...

    A. Kovařík, B. Koukalová, K. Y. Lim, R. Matyášek, C. P. Lichtenstein… in Chromosome Research (2000)

  9. No Access

    Article

    Map** of 5-methylcytosine residues in Nicotiana tabacum 5S rRNA genes by genomic sequencing

    Genomic sequencing was used to localise 5-methylcytosine residues in individual DNA strands of 5S rRNA genes in tobacco. The density of methylation along the sequence was high in both strands, exceeding the a...

    J. Fulneček, R. Matyášek, A. Kovařík, M. Bezděk in Molecular and General Genetics MGG (1998)

  10. No Access

    Article

    Meiotic transmission of a hypomethylated repetitive DNA family in tobacco

    We have recently shown that hypomethylation of cytosine residues in the HRS60 family of repetitive DNA sequences can be induced with 5-azacytidine (5-azaC) in tobacco tissue cultures. We have also proven that ...

    B. Vyskot, B. Koukalová, A. Kovařík, L. Sachambula… in Theoretical and Applied Genetics (1995)

  11. No Access

    Article

    Session 01 Cell structure and development

    M. De Agazio, R. Federico, E. Rea, A. Ciofi-Luzzatto, M. L. Zaccaria… in Biologia Plantarum (1994)

  12. Article

    Immunological quantification of recA protein in cell extracts ofE. coli after exposure to chemical mutagens or UV radiation

    Increased synthesis of RecA protein is induced inE. coli cells after their damage, the rate of synthesis being dependent on the extent of DNA alterations. The level of the RecA protein was determined inE. coli ce...

    I. Fridrichová, A. Kovařík, O. Rosskopfová in Folia Microbiologica (1992)

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  13. Article

    No significant correlation between specific antibodies to mouse mammary tumour virus and human cancer

    To study the possible involvement of mouse mammary tumour virus (MMTV) related agent in human cancer we analysed 300 samples of human sera for the presence of antibodies to MMTV structural proteins. All sera w...

    A Kovařík, K Hlubinová, J Prachař, D Šimkovič, J Knotek in British Journal of Cancer (1989)

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