100 Result(s)
within
Protocol
Yi** Qi
Life Sciences
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Protocol
Protein Phosphatase Type 1 and Type 2A Assays
Protein phosphatases type 1 (PP1) and type 2A (PP2A) are the only activities known in mammalian tissues to dephosphorylate glycogen phosphorylase a. Phosphorylase was the first enzyme demonstrated to undergo regu...
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Expression of Heterologous Integrin Genes
Cell-extracellular matrix interactions are mediated predominantly by integrins, cell-surface receptors that exist as heterodimers of noncovalently associated α and β subunits (1). Integrins have been implicated i...
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Functional Analysis of Cytoskeletal Components in the Develo** Visual System of Drosophila
A central issue in neuroscience is to understand the mechanisms underlining the stereotyped patterns of neuronal connectivity. Studies of neuronal development in both vertebrates and invertebrates have establi...
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Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture
The availability of murine embryonic stem (ES) cells has revolutionized the study of mammalian development and disease. We recently developed a culture medium that has enabled us to identify the essential sign...
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μParaflo™ Biochip for Nucleic Acid and Protein Analysis
We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using ...
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Construction of Infectious Clones of Double-Stranded DNA Viruses of Plants Using Citrus Yellow Mosaic Virus as an Example
Double-stranded DNA (dsDNA) viruses of plants are believed to be plant pararetroviruses. Their genome is replicated by reverse transcription of a larger than unit-length terminally redundant RNA transcript of ...
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Analysis of Microdissected Cells by Two-Dimensional LC-MS Approaches
Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. We combined the ...
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Isolation and Primary Culture of Human Proximal Tubule Cells
Primary cultures of renal proximal tubule cells (PTC) have been widely used to investigate tubule cell function. They provide a model system where confounding influences of renal haemodynamics, cell heterogene...
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Qualitative and Quantitative Analysis of Peptide Microarray Binding Experiments Using SVM-PEPARRAY
A main objective of analyzing peptide array-based binding experiments is to uncover the relationship between a peptide sequence and the binding outcome. Limited by the peptide array technologies available for ...
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Measurement of Glomerular Filtration Rate in Conscious Mice
Glomerular filtration rate (GFR) is an important index of renal function and routinely used in patient care and basic research to evaluate progression of renal diseases or test the efficacy of novel therapeuti...
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Web-Based Design of Peptide Microarrays Using μPepArray Pro
Peptide microarrays (peptide arrays) have increasingly become an important research tool for studying protein detection, profiling, and protein–protein interactions, and they have the potential to foster high-...
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Bacterial Delivery of siRNAs: A New Approach to Solid Tumor Therapy
RNAi is a powerful research tool for specific gene silencing and may also lead to promising novel therapeutic strategies. However, the development of RNAi-based therapies has been slow due to the lack of targe...
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Purification of Arabidopsis Argonaute Complexes and Associated Small RNAs
Argonaute (AGO) proteins recruit small RNAs to form effector complexes of RNA interference (RNAi), collectively termed RNA-induced silencing complexes (RISCs). Here, we describe detailed protocols for the puri...
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Characterization of Anti-competitor Activities Produced by Oral Bacteria
Most bacteria in nature exist in multispecies communities known as biofilms. In the natural habitat where resources (nutrient, space, etc.) are usually limited, individual species must compete or collaborate w...
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Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila
Using Drosophila actin-binding protein Dunc-115 as an example, this chapter describes an MARCM based method for analyzing cytoskeletal components for their functions in the nervous system. Following a concise des...
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Procedures for Somatic Cell Nuclear Transfer in the Rat
Somatic cell nuclear transfer (SCNT) is a powerful tool for the investigation of the mechanisms of nuclear remodeling. In addition, SCNT may offer the possibility of introducing targeted mutations by homologou...
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Computational Identification of Plant Transcription Factors and the Construction of the PlantTFDB Database
Transcription factors (TFs) play an important role in gene regulation. Computational identification and annotation of TFs at genome scale are the first step toward understanding the mechanism of gene expressio...
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Gene Therapy of Salivary Diseases
For many years, our laboratory has been develo** gene transfer approaches for salivary gland disorders that currently lack effective therapy. The purpose of this chapter is to describe key methods used in th...
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Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag
Plant disease resistance (R) proteins confer strong resistance against pathogens by recognizing particular pathogen effectors. Identification of proteins associated with an R protein will provide insight into ...
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Approaches to the Identification of ABAR as an Abscisic Acid Receptor
Abscisic acid (ABA) is a vital phytohormone that regulates seed maturation and germination, seedling growth, and adaptation to environmental stresses. ABA functions through a complex network of signaling pathw...
