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Protocol
Methods to Study Antagonistic Activities Among Oral Bacteria
Most bacteria in nature exist in multispecies communities known as biofilms. In the natural habitat where resources (nutrient, space, etc.) are usually limited, individual species must compete or collaborate w...
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Article
Open AccessMutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleTJE
The cytochrome P450 enzyme OleTJE from Jeotgalicoccus sp. ATCC 8456 is capable of converting free long-chain fatty acids into α-alkenes via one-step oxidative decarboxylation in presence of H2O2 as cofactor or us...
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Article
In vitro reconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins
The cytochrome P450 enzymes (CYPs) CYP-sb21 from Sebekia benihana and CYP-pa1 from Pseudonocardia autotrophica are able to hydroxylate the immunosuppressant cyclosporin A (CsA) in a regioselective manner, giving ...
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Protocol
Methods to Study Antagonistic Activities Among Oral Bacteria
Most bacteria in nature exist in multispecies communities known as biofilms. In the natural habitat where resources (nutrient, space, etc.) are usually limited, individual species must compete or collaborate w...
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Article
Open AccessImproved production of fatty alcohols in cyanobacteria by metabolic engineering
Fatty alcohols are widely used in industrial chemicals. The biosynthetic pathways for fatty alcohols are diverse and widely existing in nature. They display a high capacity to produce fatty alcohols by the met...
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Article
Construction, characterization and application of molecular tools for metabolic engineering of Synechocystis sp.
An integrative gene expression system has been constructed for the directional assembly of biological components in Synechocystis PCC6803. We have characterized 11 promoter parts with various expression efficienc...
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Protocol
Characterization of Anti-competitor Activities Produced by Oral Bacteria
Most bacteria in nature exist in multispecies communities known as biofilms. In the natural habitat where resources (nutrient, space, etc.) are usually limited, individual species must compete or collaborate w...
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Article
Open AccessSpecific Binding and Mineralization of Calcified Surfaces by Small Peptides
Several small (<25aa) peptides have been designed based on the sequence of the dentin phosphoprotein, one of the major noncollagenous proteins thought to be involved in the mineralization of the dentin extrace...
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Chapter
Quorum Sensing in Streptococci
Emerging studies aimed at understanding the molecular underpinnings of streptococcal infections highlight the importance of quorum sensing (QS) for biofilm formation and virulence in many ...
