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    Protocol

    Bone Marrow and Fetal Liver Radiation Chimeras

    Radiation chimeras are prepared by subjecting recipient mice to sublethal or lethal dose of irradiation and injecting them with hematopoietic stem cells (HSC) from untreated donor mice. HSC can be obtained fro...

    Francis A. Flomerfelt, Ronald E. Gress in T-Cell Development (2016)

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    Protocol

    Analysis of Cell Proliferation and Homeostasis Using EdU Labeling

    Determination of cellular proliferation and population turnover is an important tool for research on lymphoid cell function. Historically this has been done using radiolabeled nucleotides or nucleoside analogs...

    Francis A. Flomerfelt, Ronald E. Gress in T-Cell Development (2016)

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    Protocol

    Thymic Involution

    The thymus contributes to the regulation of tolerance and the prevention of autoimmunity at many levels. First, auto-reactive CD4+ and CD8+ T cells are clonally deleted during negative selection in the thymus, es...

    Frances T. Hakim, Ronald E. Gress in Immunological Tolerance (2007)