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Article
Open AccessIntegration of regulatory signals through involvement of multiple global regulators: control of the Escherichia coli gltBDF operon by Lrp, IHF, Crp, and ArgR
The glutamate synthase operon (gltBDF) contributes to one of the two main pathways of ammonia assimilation in Escherichia coli. Of the seven most-global regulators, together affecting expression of about half of ...
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Article
Effector regulation in a monomeric enzyme
The monomeric B12-dependent ribonucleotide reductase from L. leichmannii has the central 10-stranded α/β-barrel found in all ribonucleotide reductases but incorporates two distinctive structural features, a novel...
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Article
Domain alternation switches B12-dependent methionine synthase to the activation conformation
B12-dependent methionine synthase (MetH) from Escherichia coli is a large modular protein that uses bound cobalamin as an intermediate methyl carrier. Major domain rearrangements have been postulated to explain h...
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Article
The structure and properties of methylenetetrahydrofolate reductase from Escherichia coli suggest how folate ameliorates human hyperhomocysteinemia
Elevated plasma homocysteine levels are associated with increased risk for cardiovascular disease and neural tube defects in humans. Folate treatment decreases homocysteine levels and dramatically reduces the ...
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Article
Human methylenetetrahydrofolate reductase: isolation of cDNA, map** and mutation identification
Methylenetetrahydrofolate reductase (MTHFR) catalyses the reduction of methylenetetrahydrofolate to methyltetrahydrofolate, a cofactor for homocysteine methylation to methionine. MTHFR deficiency, an autosomal...