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  1. No Access

    Article

    Analysis of the promoter region in the rrnA operon from a blue-green alga, Anacystis nidulans 6301

    The nucleotide sequence has been determined of a 1431 base pair (bp) region upstream from the 16S rRNA gene in the rrnA operon of a blue-green alga, Anacystis nidulans 6301. The transcriptional initiation site ha...

    Masanobu Kumano, Noboru Tomioka, Kazuo Shinozaki… in Molecular and General Genetics MGG (1986)

  2. No Access

    Article

    Tobacco chloroplast gene coding for subunit I of proton-translocating ATPase: comparison with the wheat subunit I and E. coli subunit b

    The tobacco chloroplast gene for subunit I of proton-translocating ATPase is located 405 by downstream from the gene for subunit III and 58 by upstream from the gene for subunit a in the same DNA strand. This ...

    Kazuo Shinozaki, Hiroshi Deno, Tatsuya Wakasugi, Masahiro Sugiura in Current Genetics (1986)

  3. No Access

    Article

    Intron in the gene for the ribosomal protein S16 of tobacco chloroplast and its conserved boundary sequences

    The nucleotide sequence of a tobacco chloroplast gene coding for a protein homologous to Escherichia coli ribosomal protein S16 (rps16) has been determined. It is located 690 bp upstream from the gene for tRNALys

    Kazuo Shinozaki, Hiroshi Deno, Mamoru Sugita… in Molecular and General Genetics MGG (1986)

  4. No Access

    Article

    Genes for the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase constitute a single operon in a cyanobacterium Anacystis nidulans 6301

    The structure and transcription of the genes for the large (LS) and small subunits (SS) of ribulose-1,5-bisphosphate carboxylase/oxygenase of Anacystis nidulans 6301 were studied. The SS gene is located 93 base p...

    Kazuo Shinozaki, Masahiro Sugiura in Molecular and General Genetics MGG (1985)

  5. No Access

    Article

    Gene organization of chloroplast DNA from the broad bean Vicia faba

    Six protein genes have been mapped on broad bean chloroplast DNA by Southern hybridization using the tobacco chloroplast genes as probes. In broad bean chloroplast DNA, the genes for the β and ε subunits of pr...

    Kazuo Shinozaki, Chong-Rong Sun, Masahiro Sugiura in Molecular and General Genetics MGG (1984)

  6. No Access

    Article

    Location and nucleotide sequence of a tobacco chlorophlast DNA segment capable of replication in yeast

    A 1.7 Kbp EcoRI fragment of Nicotiana tabacum chloroplast DNA cloned in YIp5, consisting of pBR322 and the yeast ura3 gene, possessed ars (autonomously replicating sequences) activity in Saccharomyces cerevisiae....

    Takeshi Ohtani, Hirofumi Uchimiya, Akira Kato… in Molecular and General Genetics MGG (1984)

  7. No Access

    Article

    Nucleotide sequence and transcription of the gene for the 32,000 dalton thylakoid membrane protein from Nicotiana tabacum

    The nucleotide sequence of the gene for the 32,000 dalton thylakoid membrane protein from Nicotiana tabacum has been determined. This gene is located 535 base pairs (bp) away from the inverted repeat on the right...

    Mamoru Sugita, Masahiro Sugiura in Molecular and General Genetics MGG (1984)

  8. No Access

    Article

    Molecular heterogeneity in mitochondrial and chloroplast DNAs from normal and male sterile cytoplasms in sugar beets

    Mitochondrial (mt) and chloroplast (ct) DNAs were prepared from normal (N) and male sterile (S) cytoplasmic lines of sugar beet. The DNAs were cleaved with BamHI, EcoRI, HindIII and SalI enzymes, and the resultan...

    Tetsuo Mikami, Masahiro Sugiura, Toshiro Kinoshita in Current Genetics (1984)

  9. No Access

    Article

    Sequence analysis of the junctions between a large inverted repeat and single-copy regions in tobacco chloroplast DNA

    Tobacco chloroplast DNA contains a large inverted repeat sequence of 26 kilobase pairs (kbp). The inverted repeat is separated by 20 kbp small single-copy and 90 kbp large single-copy regions. We have cloned f...

    Mamoru Sugita, Akira Kato, Hiroaki Shimada… in Molecular and General Genetics MGG (1984)

  10. No Access

    Article

    Nucleotide sequence of the 16S-23S spacer region in the rrnA operon from a blue-green alga, Anacystis nidulans

    The nucleotide sequence of an entire spacer region between the 16S and 23S rRNA genes of the rrnA operon from a blue-green alga, Anacystis nidulans, has been determined. The spacer region is 545 base pairs long a...

    Noboru Tomioka, Masahiro Sugiura in Molecular and General Genetics MGG (1984)

  11. No Access

    Article

    Molecular cloning of tobacco chromosomal and chloroplast DNA segments capable of replication in yeast

    An autonomously replicating sequence (ars) of Nicotiana tabacum was cloned into the EcoRI site of YIp5, which consists of pBR322 and the yeast ura3 gene. Recombinant DNA was capable of transforming ura3- Saccarom...

    Hirofumi Uchimiya, Takeshi Ohtani, Toshifumi Ohgawara… in Molecular and General Genetics MGG (1983)

  12. No Access

    Article

    The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga, Anacystis nidulans

    The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga, Anacystis nidulans, has been determined. Its coding region is estimated to be 1,487 base pairs long, which is nearly identical ...

    Noboru Tomioka, Masahiro Sugiura in Molecular and General Genetics MGG (1983)

  13. No Access

    Article

    Physical map**s of chloroplast DNA from liverwort Marchantia polymorpha L. cell suspension cultures

    Physical maps of liverwort Marchantia polymorpha chloroplast DNA were constructed with restriction endonucleases, BamHI, SmaI, KpnI, and XhoI. The molecular size, calculated from the sum of the restriction fragme...

    Kanji Ohyama, Yoshiaki Yamano, Hideya Fukuzawa… in Molecular and General Genetics MGG (1983)

  14. No Access

    Article

    Molecular cloning and characterization of ribosomal RNA genes from a blue-green alga, Anacystis nidulans

    Two PstI fragments (5.3x106 and 4.3x106 daltons) coding for Anacystis nidulans rRNA genes were cloned. The cloned rDNAs were characterized by restriction endonuclease map**, DNA-RNA hybridization analysis and ...

    Noboru Tomioka, Kazuo Shinozaki, Masahiro Sugiura in Molecular and General Genetics MGG (1981)

  15. No Access

    Article

    Heterogeneity of 5S RNA species in tobacco chloroplasts

    Tobacco chloroplasts were found to contain three species of 5S RNA with different electrophoretic mobility. The nucleotide sequences of two species of the 5S RNA have been determined. The large 5S RNA species ...

    Fumio Takaiwa, Masahiro Sugiura in Molecular and General Genetics MGG (1981)

  16. No Access

    Article

    Nucleotide sequences of the 4.5 S and 5 S ribosomal RNA genes from tobacco chloroplasts

    The DNA sequences of the 4.5 S and 5 S RNA genes from tobacco chloroplasts have been determined. The coding regions for the mature 4.5 S and 5 S RNAs were identified by sequencing these RNAs. The 4.5 S and 5 S...

    Fumio Takaiwa, Masahiro Sugiura in Molecular and General Genetics MGG (1980)

  17. No Access

    Article

    Existence of the gene for 4.55 RNA in broad bean chloroplast DNA

    Tobacco chloroplast 4.5S RNA hybridized specifically to the 1.16 × 106 dalton Eco RI fragment of broad bean chloroplast DNA. This indicates the existence of the gene for 4.5S RNA in broad bean chloroplasts which ...

    Masahiro Sugiura in Current Genetics (1980)

  18. No Access

    Article

    Characterization of the cloned ribosomal DNA of tobacco chloroplasts

    A physical map of the cloned ribosomal DNA of tobacco chloroplasts was constructed by cleavage with several restriction endonucleases and hybridization of the restriction fragments with labeled 16S and 23S rRN...

    Jun Kusuda, Kazuo Shinozaki, Fumio Takaiwa… in Molecular and General Genetics MGG (1980)

  19. No Access

    Article

    Molecular cloning of tobacco chloroplast ribosomal RNA genes

    Tobacco chloroplast ribosomal RNAs were shown to be hybridized with two EcoRI fragments of tobacco chloroplast DNA. These DNA fragments having molecular weights of 1.9x106 and 2.8x106 daltons were cloned using th...

    Masahiro Sugiura, Jun Kusuda in Molecular and General Genetics MGG (1979)

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