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Chapter
Potato Genome Editing: Recent Challenges and a Practical Procedure
Potato (Solanum tuberosum L.) is one of the most important staple crops, accounting for the fourth highest production in the world. To date, many studies on potatoes using genome-editing technologies have been re...
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Article
Changes in Radixin Expression and Interaction with Efflux Transporters in the Liver of Adjuvant-Induced Arthritic Rats
Scaffold proteins such as radixin help to modulate the plasma membrane localization and transport activity of the multidrug resistance-associated protein 2 (MRP2/ABCC2) and P-glycoprotein (P-gp/ABCB1) efflux t...
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Article
Open AccessEstablishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato
CRISPR/Cas9 is a programmable nuclease composed of the Cas9 protein and a guide RNA (gRNA) molecule. To create a mutant potato, a powerful genome-editing system was required because potato has a tetraploid gen...
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Article
Open AccessA novel role for OATP2A1/SLCO2A1 in a murine model of colon cancer
Prostaglandin E2 (PGE2) is associated with proliferation and angiogenesis in colorectal tumours. The role of prostaglandin transporter OATP2A1/SLCO2A1 in colon cancer tumorogenesis is unknown. We evaluated mice o...
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Article
Open AccessA simple Gateway-assisted construction system of TALEN genes for plant genome editing
TALEN is an artificial nuclease being applied for sequence-specific genome editing. For the plant genome editing, a pair of TALEN genes is expressed in the cells and a binary plasmid for Agrobacterium-mediated tr...
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Article
High-resolution genetic map** and physical map construction for the fertility restorer Rfm1 locus in barley
High-resolution genetic linkage map** and BAC physical map** narrowed the fertility restorer locus Rfm1 in barley to a sub-centimorgan genetic interval ...
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Article
Acquired resistance to the rice blast in transgenic rice accumulating the antimicrobial peptide thanatin
Thanatin is an antimicrobial peptide with a strong and wide-ranging antimicrobial spectrum, including certain species of fungi and Gram-negative and Gram-positive bacteria. To evaluate the application of thana...
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Article
Simple construction of plant RNAi vectors using long oligonucleotides
RNA interference (RNAi) is one of the most important technologies currently available for the analysis of gene function. However, despite the development of various methods, it is still difficult to construct ...
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Chapter and Conference Paper
Assessment of Safety on Evacuating Route During Underground Flooding
Heavy rainfalls over 50 mm/hr have been observed frequently in the last decade in Japan. If a sewage system in urban area could not drain such rains, flood water would be flooding and flowing into underground ...
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Article
Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase λ and RPA subunit proteins, is involved in cell proliferation
Exonuclease 1, a class III member of the RAD2 nuclease family, is a structure-specific nuclease involved in DNA metabolism (replication, repair and recombination). We have identified a homologue to Exonuclease-1 ...
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Article
Long-distance signals positively regulate the expression of iron uptake genes in tobacco roots
Long-distance signals generated in shoots are thought to be associated with the regulation of iron uptake from roots; however, the signaling mechanism is still unknown. To elucidate whether the signal regulate...
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Article
Synthesis of Short-chain-length/Medium-chain-length Polyhydroxyalkanoate (PHA) Copolymers in Peroxisome of the Transgenic Arabidopsis Thaliana Harboring the PHA Synthase Gene from Pseudomonas sp. 61-3
In this paper, the photosynthetic production of short-chain-length/medium-chain-length polyhydroxyalkanoate (PHA) copolymers is reported. The wild-type and highly active doubly mutated PHA synthase 1 (S325T/Q4...
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Article
Cadmium inducible Fe deficiency responses observed from macro and molecular views in tobacco plants
Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 μM of Cd exposure reduced the total dry weight and chlorophyll index of the se...
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Article
Molecular characterization of ONAC300, a novel NAC gene specifically expressed at early stages in various develo** tissues of rice
Members of the plant-specific gene family referred to as the NAC family (for NAM-ATAF-CUC-related) are involved in various functions including the regulation of plant development. However, no detailed molecula...
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Article
Induction of callus from a metal hypertolerant fern, Athyrium yokoscense, and evaluation of its cadmium tolerance and accumulation capacity
The callus of a metal hypertolerant fern, Athyrium yokoscense, was induced from the spores generated on a small sectioned frond in vitro. The callus grew vigorously with the periodical medium change, especially i...
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Article
Mobilization of transposons by a mutation abolishing full DNA methylation in Arabidopsis
A major component of the large genomes of higher plants and vertebrates comprises transposable elements and their derivatives, which potentially reduce the stability of the genome1. It has been proposed that meth...
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Article
A rapid PCR-aided selection of a rice line containing the Rf-1 gene which is involved in restoration of the cytoplasmic male sterility
Cytoplasmic male sterility (CMS) is widely known in higher plants, the mechanism of which is believed to involve incompatibility between nuclei and cytoplasms. In rice lines with the CMS trait, fertility is re...
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Article
High-frequency inter-parental recombination between mitochondrial genomes of rice cybrids
Analyzing more than 100 independent rice cybrids, we found evidence for inter-molecular recombination between parental mitochondrial genomes occurring at high frequency soon after protoplast fusion. The struct...
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Article
A unique sequence located downstream from the rice mitochondrialatp6 may cause male sterility
Asymmetric cell-fusion of the japonica cultivar ofOryza sativa (rice) with cytoplasmic-male-sterile (CMS) plants bearing cytoplasm derived from Chinsurah Boro II, resulted in two classes of cytoplasmic hybrids (c...
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Article
Molecular analysis of the gene encoding a rice starch branching enzyme
The sequence of a rice gene encoding a starch branching enzyme (sbe1) shows extreme divergence from that of the rice gene, that is homologous to bacterial glycogen branching enzyme (sbe2). sbe1 is expressed abund...
