Summary
Tobacco chloroplasts were found to contain three species of 5S RNA with different electrophoretic mobility. The nucleotide sequences of two species of the 5S RNA have been determined. The large 5S RNA species (5S RNAL) is composed of 121 nucleotides and the small 5S RNA species (5S RNAs) of 119 nucleotides. The 5S RNAL contains and extra uridine residue at both 5′ and 3′ends of the 5S RNAs.
Similar content being viewed by others
References
Brownlee GG, Sanger F, Barrell BG (1968) The sequence of 5S ribosomal ribonucleic acid. J Mol Biol 34:379–412
Corry MJ, Payne PI, Dyer TA (1974) The nucleotide sequence of 5S rRNA from the blue-green alga Anacystis nidulans. FEBS Lett 46:63–66
Donis-Keller H, Maxam AM, Gilbert W (1977) Map** adenines, guanines, and pyrimidines in RNA. Nucleic Acids 4:2527–2538
DuBuy B, Weissman SM (1971) Nucleotide sequence of Pseudomonas fluorescens 5S ribonucleic acid. J Biol Chem 246:747–761
Dyer TA, Bowman CM (1979) Nucleotide sequence of chloroplast 5S ribosomal ribonucleic acid in flowering plants. Biochem J 183:595–604
Hori H (1976) Molecular evolution of 5S RNA. Mol Gen Genet 145:119–123
Jarry B, Rosset R (1971) Heterogeneity of 5S RNA in Escherichia coli. Mol Gen Genet 113:43–50
Jurgenson JE, Bourque DP (1980) Map** of rRNA genes in an inverted repeat in Nicotiana tabacum chloroplast DNA. Nucleic Acids 8:3505–3516
Kimura M, Ohta T (1973) Eukaryotes-prokaryotes divergence estimated by 5S ribosomal RNA sequence. Nature New Biol 243:199–200
Lockard RE, Alzner-Deweerd B, Heckman JE, MacGee J, Tabor MW, RajBhandary UL (1978) Sequence analysis of 5 [32P]labeled mRNA and tRNA using polyacrylamide gel electrophoresis. Nucleic Acids 5:37–56
Margulis L (1970) Origin of eukaryotic cells. Yale Univ Press, New Haven
Payne PI, Dyer TA (1976) Evidence for the nucleotide sequence of 5S rRNA from the flowering plant Secale cereale (Rye). Eur J Biochem 71:33–38
Peattie DA (1979) Direct chemical method for sequencing RNA. Proc Natl Acad Sci USA 76:1760–1764
Raué HA, Heerschap A, Planta RJ (1976) Occurrence in Bacillus licheniformis of two species of 5S RNA with multiple differences in primary structure. Eur J Biochem 68:169–176
Raué HA, Rosner A, Planta RJ (1977) Heterogeneity of the gene coding for 5S RNA in three related strains of genus Bacillus. Mol Gen Genet 156:185–193
Simoncsits A, Brownlee GG, Brown RS, Rubin JR, Guilley H (1977) New rapid gel sequencing method for RNA. Nature 269:833–836
Sugiura M, Kusuda J (1979) Molecular cloning of tobacco chloroplast ribosomal RNA genes. Mol Gen Genet 172:137–141
Sugiura M, Suzuki M, Ohtsuka E, Nishikawa S, Uemura H, Ikehara M (1979) Purification of T4 RNA ligase by 2′, 5′-ADP sepharose chromatography. FEBS Lett 97:73–76
Sugiura M, Takanami M (1967) Analysis of the 5′-terminal nucleotide sequences of ribonucleic acids. Proc Natl Acad Sci USA 58:1595–1602
Takaiwa F, Suguira M (1980a) Cloning and characterization of 4.5S and 5S RNA genes in tobacco chloroplasts. Gene 10:95–103
Takaiwa F, Sugiura M (1980b) Nucleotide sequences of the 4.5S and 5S ribosomal RNA genes from tobacco chloroplasts. Mol Gen Genet (in press)
Whitfeld PR (1977) Chloroplast RNA. In: Stewart PR, Letham DS (eds) Teh ribonucleic acids. Springer-Verlag, Berlin-Heidelberg, 2nd ed, p 297
Author information
Authors and Affiliations
Additional information
Communicated by J. Schell
Rights and permissions
About this article
Cite this article
Takaiwa, F., Sugiura, M. Heterogeneity of 5S RNA species in tobacco chloroplasts. Molec. Gen. Genet. 182, 385–389 (1981). https://doi.org/10.1007/BF00293926
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00293926