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Article
Open AccessPhotoisomerization pathway of the microbial rhodopsin chromophore in solution
Photoisomerization is a key photochemical reaction in microbial and animal rhodopsins. It is well established that such photoisomerization is highly selective; all-trans to 13-cis, and 11-cis to all-trans forms i...
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Article
PBRM1-deficient PBAF complexes target aberrant genomic loci to activate the NF-κB pathway in clear cell renal cell carcinoma
PBRM1 encodes an accessory subunit of the PBAF SWI/SNF chromatin remodeller, and the inactivation of PBRM1 is a frequent event in kidney cancer. However, the impact of PBRM1 loss on chromatin remodelling is not w...
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Article
Rhodopsin-bestrophin fusion proteins from unicellular algae form gigantic pentameric ion channels
Many organisms sense light using rhodopsins, photoreceptive proteins containing a retinal chromophore. Here we report the discovery, structure and biophysical characterization of bestrhodopsins, a microbial rh...
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Article
Open AccessThe heavy chain of 4F2 antigen promote prostate cancer progression via SKP-2
The 4F2 cell-surface antigen heavy chain (4F2hc) forms a heterodimeric complex with L-type amino acid transporter 1 (LAT1) and transports large neutral essential amino acids. However, in contrast to the tradit...
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Chapter
Molecular Properties and Optogenetic Applications of Enzymerhodopsins
The cyclic nucleotides cAMP and cGMP are ubiquitous secondary messengers that regulate multiple biological functions including gene expression, differentiation, proliferation, and cell survival. In sensory neu...
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Article
Open AccessStructural insights into the mechanism of rhodopsin phosphodiesterase
Rhodopsin phosphodiesterase (Rh-PDE) is an enzyme rhodopsin belonging to a recently discovered class of microbial rhodopsins with light-dependent enzymatic activity. Rh-PDE consists of the N-terminal rhodopsin...
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Article
Open AccessExpression of L-type amino acid transporter 1 as a molecular target for prognostic and therapeutic indicators in bladder carcinoma
L-type amino acid transporter 1 (LAT1) plays a role in transporting essential amino acids including leucine, which regulates the mTOR signaling pathway. Here, we studied the expression profile and functional r...
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Article
Processing of the 5′-UTR and existence of protein factors that regulate translation of tobacco chloroplast psbN mRNA
The chloroplast psbB operon includes five genes encoding photosystem II and cytochrome b 6 /f complex components. The psbN gene is located on the opposite strand. PsbN is localized...
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Protocol
Plastid mRNA Translation
Overall translational machinery in plastids is similar to that of E. coli. Initiation is the crucial step for translation and this step in plastids is somewhat different from that of E. coli. Unlike the Shine-Dal...
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Chapter
Translation in Chloroplasts of Flowering Plants
The chloroplast genome in flowering plants contains about 80 protein-coding genes. The chloroplast translational machinery, which is similar to that of Escherichia coli, reads the corresponding mRNAs. Translation...
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Article
Enantioselective Hydrogenation of Olefins using Commercially Available Pd/C. Chiral Heterogeneous Catalyst Applicable for High-Throughput Screening
Cinchonidine-modified Pd/C applicable for the high-throughput-guided study was developed. Commercial Pd/C catalysts were employed for the enantioselective hydrogenation of phenylcinnamic acid after the pretrea...
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Article
XAFS and XRD Analysis of Ceria–Zirconia Oxygen Storage Promoters for Automotive Catalysts
Three types of CeO2–ZrO2 (Ce:Zr = 1:1 molar ratio) compounds with different oxygen storage/release capacity (OSC) were characterized by means of the Ce K-edge and Zr K-edge XAFS. In order to investigate the relat...
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Chapter
RNA Editing in Chloroplasts
RNA editing has been observed in chloroplast transcripts from most land plants, but not from algae. In higher plant chloroplasts, C-to-U conversion occurs at around 30 specific sites in mRNAs, many of which re...
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Article
The complete structure of the cucumber (Cucumis sativus L.) chloroplast genome: Its composition and comparative analysis
The complete nucleotide sequence of the cucumber (C. sativus L. var. Borszczagowski) chloroplast genome has been determined. The genome is composed of 155,293 bp containing a pair of inverted repeats of 25,191 bp...
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Article
Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization
The entire genome of the unicellular cyanobacterium Synechococcus elongatus PCC 6301 (formerly Anacystis nidulans Berkeley strain 6301) was sequenced. The genome consisted of a circular chromosome 2,696,255 bp lo...
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Article
The 2005 version of the chloroplast DNA sequence from tobacco (Nicotiana tabacum)
The complete nucleotide sequence of tobacco chloroplast DNA was first determined in 1986, and then its updated gene map was reported in 1998. During the course of sequencing the chloroplast DNA ofNicotiana sylves...
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Chapter
History of chloroplast genomics
The presence of chloroplast DNA was established in 1963. With the development of recombinant DNA technologies, chloroplast DNA was selected as one of the first candidates for genome sequencing. The first physi...
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Article
Comparative NO x reduction behavior of Pt, Pd, and Rh supported catalysts in simulated exhaust gases as a function of oxygen concentration
NO x reduction activity on Pt and Pd catalysts had a maximum for S value as stoichiometry number at a fixed temperature, and the S value at the maximum NO x ...
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Protocol
In Vitro RNA Editing Systems From Higher Plant Chloroplasts
RNA editing in higher plant chloroplasts involves C-to-U conversion at ∼30 specific sites. In vitro systems supporting accurate editing have been developed from tobacco and pea chloroplasts. mRNA substrates la...
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Chapter
In Vitro Transcription Systems from BY-2 Cells
The in vitro transcription system is a powerful method to dissect molecular mechanisms of transcription of genes in question, usually performed in cellular or nuclear extracts containing transcription apparatu...
