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A Time and Cost-Effective Pipeline for Expression Screening and Protein Production in Insect Cells Based on the HR-Bac Toolbox to Generate Recombinant Baculoviruses
The baculovirus expression vector system (BEVS) is recognized as a powerful platform for producing challenging proteins and multiprotein complexes...
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Probing Baculovirus Vector Gene Essentiality for Foreign Gene Expression Using a CRISPR-Cas9 System
The baculovirus expression vector system (BEVS) has now found acceptance in both research laboratories and industry, which can be attributed to many...
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HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system
The Baculovirus/insect cell expression system is a powerful technology for reconstitution of eukaryotic macromolecular assemblies. Most multigene...
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Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS)
The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our...
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TOPO Cloning for Efficient Plasmid Construction
This chapter outlines the use of TOPO cloning for streamlined generation of a recombinant plasmid containing your gene of interest for use in the...
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The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production
Baculovirus-mediated gene expression in mammalian cells, BacMam, is a useful alternative to transient transfection for recombinant protein production...
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Production of Recombinant Viral Antigens Using the Baculovirus-Insect Cell Expression System
Insect cell expression has been successfully used for the production of viral antigens as part of commercial vaccine development. As expression host,...
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Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System
Mammalian cell lines are one of the best options when it comes to the production of complex proteins requiring specific glycosylation patterns....
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Functional Baculovirus Particle Quantification via Plaque Assay
Plaque assay method enables the quantification of infectious baculovirus when defined as plaque forming units (PFU). It allows to determine the...
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Expression and Purification of Inflammasome Sensor NOD-Like Receptor Protein-1 Using the Baculovirus-Insect Cell Expression System
Inflammasomes are innate immune sensing and signaling complexes critical for defense against pathogens and response to cellular stresses. A core...
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Recombinant AAV Production
The insect cell-baculovirus expression vector (IC-BEV) platform has enabled small research-scale and large commercial-scale production of recombinant...
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Overexpression of Endoplasmic Reticulum Proteins from Arabidopsis thaliana in Baculovirus
The overproduction of proteins of the endoplasmic reticulum (ER) of plant cells in prokaryotic heterologous gene expression system remains a...
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Scarless Baculovirus Genome Editing Using Lambda-Red Recombineering in E. coli
Baculoviruses are widely used for their potential as biological pesticide and as platform for the production of recombinant proteins and gene therapy...
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Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System
The Bac-to-Bac® Baculovirus Expression System provides a rapid and efficient method to generate recombinant cryptochrome (CRY) proteins with...
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Assembly of Baculovirus Vectors for Multiplexed Prime Editing
Efficient genome editing by using CRISPR technologies requires simultaneous and efficient delivery of multiple genetically encoded components to...
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Construction and Characterization of a Novel Bacmid AcBac-Syn Based on a Synthesized Baculovirus Genome
Baculoviruses are large DNA viruses which have been widely used as expression vectors and biological insecticides. Homologous recombination and...
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Bacterial Artificial Chromosome-based Protein Expression Platform Using the Tol2 Transposon System
Conventional vector systems, including plasmid-based vectors, are mainly used in mammalian cells for the production of biopharmaceuticals....
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Production of Copepod Luciferases via Baculovirus Expression System
Secreted copepod luciferases (CopLucs) represent highly homologous enzymes which catalyze the oxidation of a low molecular weight substrate,...
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TCID50 Assay: A Simple Method to Determine Baculovirus Titer
There are many methods that can be used to determine the infectious titer of your baculovirus stock. The TCID50 method is a simple end-point dilution...
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Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System
Several vaccines are already produced using the baculovirus expression vector system (BEVS). This chapter describes methods for generating...