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Bioluminescence

Methods and Protocols, Volume 1

  • Book
  • © 2022
  • Latest edition

Overview

Editors:
  1. Sung-Bae Kim

    1. Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2524)

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About this book

This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 1 delves into techniques for the establishment of luciferins and luciferases, basic in vitro and in vivo applications, as well as protocols on multiplex imaging platforms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 1 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Establishment of Luciferins and Luciferases

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Gene Cloning and Functional Analysis of the Luciferase from Luminous Syllids of the Genus Odontosyllis

      • Rie Yasuno, Yasuo Mitani, Yoshihiro Ohmiya
      Pages 3-15

    3. Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging

      • Tianyu Jiang, Minyong Li
      Pages 17-36

    4. Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging

      • Nobuo Kitada, Shojiro Maki, Sung-Bae Kim
      Pages 37-51

    5. Biosynthesis-Inspired Deracemizative Production of D-Luciferin In Vitro by Combining Luciferase and Thioesterase

      • Kazuki Niwa, Dai-ichiro Kato
      Pages 53-58

    6. Production of Metridia Luciferase in Native Form by Oxidative Refolding from E. coli Inclusion Bodies

      • Svetlana V. Markova, Marina D. Larionova, Eugene S. Vysotski
      Pages 59-73

    7. Production of Copepod Luciferases via Baculovirus Expression System

      • Marina D. Larionova, Svetlana V. Markova, Eugene S. Vysotski
      Pages 75-89

    8. Molecular Tension Probe for In Vitro Bioassays

      • Sung-Bae Kim, Rika Fujii, Simon Miller, Mikio Tanabe
      Pages 91-103

  3. Basic In Vitro Applications

    1. Front Matter

      Pages 105-105
      Download chapter PDF

    2. Optimized Loop-Mediated Isothermal Amplification (LAMP) Allows Single Copy Detection Using Bioluminescent Assay in Real Time (BART)

      • Patrick Hardinge
      Pages 107-117

    3. A Simple and Rapid Bioluminescence-Based Functional Assay of Organic Anion Transporter 1 as a D-Luciferin Transporter

      • Katsuhisa Inoue, Koki Sugiyama, Takahito Furuya
      Pages 119-126

    4. A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules Against the Intracellular Form of Leishmania infantum

      • Diego Benítez, Andrea Medeiros, Cristina Quiroga, Marcelo A. Comini
      Pages 127-147

    5. A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening Against Infective African Trypanosomes

      • Estefania Dibello, Marcelo A. Comini, Diego Benítez
      Pages 149-162

    6. Imaging of Autonomous Bioluminescence Emission From Single Mammalian Cells

      • Carola Gregor
      Pages 163-172

    7. Rapid Single-Cell Detection of Beer-Contaminating Lactic Acid Bacteria Using Bioluminescence/Rapid Microbe Detection

      • Toshihiro Takahashi, Yasukazu Nakakita
      Pages 173-182

    8. Bioluminescence of Aliivibrio Fischeri in Artificial Seawater and Its Application in Fungicide Sensing

      • Hitomi Kuwahara, Hiroshi Morita
      Pages 183-195

    9. A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor

      • Thabat Khatib, Berndt Müller, Peter McCaffery
      Pages 197-207

    10. Design of an Intron-Retained Bioluminescence Reporter and its Application in Imaging of Pre-mRNA Splicing in Living Subjects

      • Fu Wang, Si Chen, Haifeng Zheng, Bin Guo
      Pages 209-221

    11. Generation of Bi-Reporter-Expressing Tri-Segmented Arenavirus

      • Cheng** Ye, Luis Martinez-Sobrido
      Pages 223-233
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Editors and Affiliations

  • Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

    Sung-Bae Kim

Bibliographic Information

  • Book Title: Bioluminescence

  • Book Subtitle: Methods and Protocols, Volume 1

  • Editors: Sung-Bae Kim

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2453-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2452-4Published: 14 July 2022

  • Softcover ISBN: 978-1-0716-2455-5Published: 14 July 2023

  • eBook ISBN: 978-1-0716-2453-1Published: 12 July 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XVI, 474

  • Number of Illustrations: 22 b/w illustrations, 108 illustrations in colour

  • Topics: Biochemistry, general, Materials Science, general

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