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Antibody Purification Using Affinity Chromatography
Antibodies are an integral part of many biological assays and biotherapeutics. However, the sources from which antibodies are derived frequently...
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An immobilized antibody-based affinity grid strategy for on-grid purification of target proteins enables high-resolution cryo-EM
In cryo-electron microscopy (cryo-EM), sample preparation poses a critical bottleneck, particularly for rare or fragile macromolecular assemblies and...
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Mixed-Mode Chromatography and Its Role in Monoclonal Antibody Purification
As the biopharmaceutical industry matures and embraces process intensification methodologies allied to the emergence of newer personalized medicines,...
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Production of Neutralizing Antibody
Techniques employing monoclonal antibodies (mAbs) are widely used in the initial development phase of biologics. The usefulness of mAbs in basic RA...
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Recombinant AAV Purification
Purification of rAAV is a crucial unit operation of the AAV production process. It enables the capture of AAV and removal of contaminants such as...
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Enzyme Purification
The growing importance of enzymes for the food, biotech, pharmaceutical, and cosmetics industries, among others, has increased the need for efficient...
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A workflow for the development of template-assisted membrane crystallization downstream processing for monoclonal antibody purification
Monoclonal antibodies (mAbs) are commonly used biologic drugs for the treatment of diseases such as rheumatoid arthritis, multiple sclerosis,...
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Harnessing ceramic hydroxyapatite as an effective polishing strategy to remove product- and process-related impurities in bispecific antibody purification
Bispecific antibody (bsAb), a novel therapeutic modality, provides excellent treatment efficacy, yet poses numerous challenges to downstream process...
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Preparation and identification of a single domain antibody specific for adenovirus vectors and its application to the immunoaffinity purification of adenoviruses
Adenovirus belongs to the family of Adenoviridae. As a vaccine carrier, it has high safety and stimulates the body to produce cellular immunity and...
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Antibody Batch Cloning
The antigen-binding ability of each antibody clone selected by phage display is usually initially ranked by a screening ELISA using monovalent scFv...
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Antibody Isolation in C. neoformans
The importance of humoral immunity to fungal infections remains to be elucidated. In cryptococcosis, patients that fail to generate antibodies...
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Production and Purification of Antibodies in Chinese Hamster Ovary Cells
Antibodies are versatile biological molecules with widespread applications in research and medicine. This protocol outlines the generation of...
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Production and Purification of Filovirus Glycoproteins
Ebola (EBOV) and Marburg (MARV) viruses cause hemorrhagic fever disease in humans and non-human primates (NHPs) with case-fatality rates as high as...
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Engineering Homogeneous Photoactive Antibody Fragments
Genetically encoded site-specifically incorporated noncanonical amino acids (ncAAs) have been used to modulate properties of several proteins. Here,...
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Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains
Antibody therapeutics have become a cornerstone of the pharmaceutical market due to their precise molecular targeting, favorable pharmacokinetic...
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Serum components influence antibody reactivity to glycan and DNA antigens
We previously generated three types of anti-glycan monoclonal IgM antibodies that react with certain structures on the glycans of glycosphingolipids...
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Protein–Protein Interaction: Tandem Affinity Purification in Bacteria
The discovery of protein–protein interaction networks can lead to the unveiling of protein complex(es) forming cellular machinerie(s) or reveal...
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Production and Purification of Recombinant Proteins
The growing demand for therapeutic proteins, especially monoclonal antibodies, increases the need for practical and economical processing techniques....
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Plant-Based Antibody Manufacturing
Plant-based manufacture of antibodies and other biologics is a rapidly growing area of interest to the biopharmaceutical industry. The use of plants...
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Highly Parallel Oligonucleotide Libraries Purification
In this thesis, I designed a highly parallel purification technology that increases the ratio of error-free oligos in complex oligo libraries by...