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Plant-Based Antibody Manufacturing

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Tools & Techniques of Plant Molecular Farming

Part of the book series: Concepts and Strategies in Plant Sciences ((CSPS))

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Abstract

Plant-based manufacture of antibodies and other biologics is a rapidly growing area of interest to the biopharmaceutical industry. The use of plants can significantly accelerate biologics production, with relatively lower infrastructure costs, compared with mammalian cell-based manufacturing. Improvements in genomics, bioinformatics, and genome engineering tools have contributed to the generation of more efficient and productive plant strains. Novel technological approaches have streamlined expression, extraction, and purification of biologics from these plants and resulted in biologics, which have successfully been used in clinical trials and treatment of patients. In this chapter, we discuss the progress and remaining challenges for plant-based manufacturing of biologics, with a focus on the use of Nicotiana benthamiana for production of antibodies.

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Change history

  • 06 January 2024

    A correction has been published.

Abbreviations

ADCC:

Antibody-dependent cell-mediated cytotoxicity

AEX:

Anion exchange chromatography

CAM-Cys:

Carbamidomethyl-cysteine

CFU:

Colony-forming unit

CHO:

Chinese hamster ovary cells

COVID-19:

SARS-CoV-2 virus

Cys:

Cysteine

Da:

Dalton

DF:

Diafiltration

DP:

Drug product

DS:

Drug substance

DSP:

Downstream process

ELISA:

Enzyme-linked immunosorbent assay

EU:

Enzyme units

Fc:

Constant fragment

Fuc:

Fucose

Gal:

Galactose

GlcNAC:

N-acetylglucosamine

GMP:

Good manufacturing practices

HC:

Heavy chain

HC-DNA:

Host cell DNA

HCP:

Host cell protein

HEK:

Human embryonic kidney cells

HIV:

Human immunodeficiency virus type 1

HSV:

Herpes simplex virus

icIEF:

Imaged capillary isoelectric focusing

IEX:

Ion-exchange chromatography

IV:

Intravenous

LC:

Light chain

LC-MS:

Liquid chromatography-mass spectrometry

Lys-C:

Lys-C protease

mAb:

Monoclonal antibody

Man:

Mannose

MW:

Molecular weight

NSO:

Murine myeloma cells

PD:

Pharmacodynamics

PK:

Pharmacokinetics

PMP:

Plant-made pharmaceutical

PVX:

Potato virus X

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

SEC-HPLC:

Size-exchange-high-pressure liquid chromatography

TFF:

Tangential flow filtration

TVCV:

Turnip vein clearing virus

U.S.P.:

U.S. Pharmacopeia

UF:

Ultrafiltration

US FDA:

U.S. Food and Drug Administration

USP:

Upstream process

UV:

Ultraviolet light wavelength

WHO:

World Health Organization

Xyl:

Xylose

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Author information

Authors and Affiliations

  1. KBio, Inc., Owensboro, KY, USA

    Gregory P. Pogue, Kelsi Swope, Josh Morton, Steve Hume, Krystal Hamorsky, Josh Fuqua, Joshua M. Royal, Sean Stevens & Barry Bratcher

  2. IC2 Institute, The University of Texas at Austin, Austin, TX, USA

    Gregory P. Pogue

  3. Latham BioPharm Group, Inc., Elkridge, MD, USA

    Joseph Rininger & Lauren Schoukroun-Barnes

  4. ZabBio, Inc., San Diego, CA, USA

    Michael H. Pauly, Max Brennan, Larry Zeitlin & Kevin Whaley

Authors
  1. Gregory P. Pogue
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  2. Kelsi Swope
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  3. Joseph Rininger
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  4. Lauren Schoukroun-Barnes
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  5. Josh Morton
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  6. Steve Hume
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  7. Krystal Hamorsky
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  8. Josh Fuqua
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  9. Joshua M. Royal
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  10. Michael H. Pauly
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  11. Max Brennan
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  12. Larry Zeitlin
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  13. Kevin Whaley
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  14. Sean Stevens
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  15. Barry Bratcher
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Corresponding author

Correspondence to Gregory P. Pogue .

Editor information

Editors and Affiliations

  1. Prof. Chittaranjan Kole Foundation for Science and Society, Kolkata, India

    Chittaranjan Kole

  2. ICAR-Indian Institute of Vegetable Research, Varanasi, India

    Anurag Chaurasia

  3. Department of Microbiology, Cornell University, Ithaca, NY, USA

    Kathleen L. Hefferon

  4. Berhampur University, Berhampur, India

    Jogeswar Panigrahi

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© 2023 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

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Pogue, G.P. et al. (2023). Plant-Based Antibody Manufacturing. In: Kole, C., Chaurasia, A., Hefferon, K.L., Panigrahi, J. (eds) Tools & Techniques of Plant Molecular Farming. Concepts and Strategies in Plant Sciences. Springer, Singapore. https://doi.org/10.1007/978-981-99-4859-8_7

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