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  1. Multiple-probe-assisted DNA capture and amplification for high-throughput African swine fever virus detection

    Abstract

    African swine fever (ASF) is one of the most devastating infectious diseases affecting domestic pigs and wild boar. The grave socio-economic...

    Huicong Wang, Hongru Pian, ... Zhi Zheng in Applied Microbiology and Biotechnology
    Article 28 December 2022
  2. Integrating genomic sequencing resources: an innovative perspective on recycling with universal Angiosperms353 probe sets

    Sequencing strategies have continually advanced, with high-throughput sequencing (HTS) technologies emerging as pivotal tools in plant phylogenomics....

    Xueqin Wang, Tao **ong, ... Miao Sun in Horticulture Advances
    Article Open access 29 January 2024
  3. Telomere-specific chromatin capture using a pyrrole–imidazole polyamide probe for the identification of proteins and non-coding RNAs

    Background

    Knowing chromatin components at a DNA regulatory element at any given time is essential for understanding how the element works during...

    Satoru Ide, Asuka Sasaki, ... Kazuhiro Maeshima in Epigenetics & Chromatin
    Article Open access 09 October 2021
  4. Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture

    Chromosome conformation capture (3C) methods measure the spatial proximity between DNA elements in the cell nucleus. Many methods have been developed...

    Damien J. Downes, Alastair L. Smith, ... Jim R. Hughes in Nature Protocols
    Article 04 February 2022
  5. Determining chromatin architecture with Micro Capture-C

    Micro Capture-C (MCC) is a chromatin conformation capture (3C) method for visualizing reproducible three-dimensional contacts of specified regions of...

    Joseph C. Hamley, Hangpeng Li, ... James O. J. Davies in Nature Protocols
    Article 29 March 2023
  6. Functionalized tetrahedral DNA frameworks for the capture of circulating tumor cells

    Identification and characterization of circulating tumor cells (CTCs) from blood samples of patients with cancer can help monitor parameters such as...

    Yirong Chen, Meihua Lin, ... Min Li in Nature Protocols
    Article 05 February 2024
  7. A comprehensive assessment of exome capture methods for RNA sequencing of formalin-fixed and paraffin-embedded samples

    RNA-Seq analysis of Formalin-Fixed and Paraffin-Embedded (FFPE) samples has emerged as a highly effective approach and is increasingly being used in...

    Liang Zong, Yabing Zhu, ... Sanjie Jiang in BMC Genomics
    Article Open access 15 December 2023
  8. ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

    Methods that measure the transcriptomic state of thousands of individual cells have transformed our understanding of cellular heterogeneity in...

    Prosenjit Samanta, Samuel F. Cooke, ... Adam Rosenthal in Nature Protocols
    Article 20 May 2024
  9. Revealing the Arabidopsis AtGRP7 mRNA binding proteome by specific enhanced RNA interactome capture

    Background

    The interaction of proteins with RNA in the cell is crucial to orchestrate all steps of RNA processing. RNA interactome capture (RIC)...

    Marlene Reichel, Olga Schmidt, ... Dorothee Staiger in BMC Plant Biology
    Article Open access 14 June 2024
  10. Rational probe design for efficient rRNA depletion and improved metatranscriptomic analysis of human microbiomes

    The microbiota that colonize the human gut and other tissues are dynamic, varying both in composition and functional state between individuals and...

    Asako Tan, Senthil Murugapiran, ... Daryl M. Gohl in BMC Microbiology
    Article Open access 20 October 2023
  11. A dicey situation: capture behaviours in free-ranging dice snakes

    Abstract

    When captured by a predator, the prey’s options for escape decrease dramatically; however, some species, such as dice snakes, display a wide...

    Vukašin Bjelica, Marko Anđelković, ... Ana Golubović in Behavioral Ecology and Sociobiology
    Article 22 April 2023
  12. Species-aware DNA language models capture regulatory elements and their evolution

    Background

    The rise of large-scale multi-species genome sequencing projects promises to shed new light on how genomes encode gene regulatory...

    Alexander Karollus, Johannes Hingerl, ... Julien Gagneur in Genome Biology
    Article Open access 02 April 2024
  13. Targeted Chromosome Conformation Capture (HiCap)

    Targeted chromosome conformation capture (HiCap) is an experimental method for detecting spatial interactions of genomic features such as promoters...
    Artemy Zhigulev, Pelin Sahlén in Spatial Genome Organization
    Protocol 2022
  14. Probe-based bacterial single-cell RNA sequencing predicts toxin regulation

    Clonal bacterial populations rely on transcriptional variation across individual cells to produce specialized states that increase fitness....

    Ryan McNulty, Duluxan Sritharan, ... Adam Z. Rosenthal in Nature Microbiology
    Article Open access 03 April 2023
  15. GenoBaits®WheatplusEE: a targeted capture sequencing panel for quick and accurate identification of wheat–Thinopyrum derivatives

    Key message

    A total of 90,000 capture probes derived from wheat and Thinopyrum elongatum were integrated into one chip, which served as an economical...

    **chuan Deng, **n Du, ... Wanquan Ji in Theoretical and Applied Genetics
    Article 31 January 2024
  16. DNA segment capture by Smc5/6 holocomplexes

    Three distinct structural maintenance of chromosomes (SMC) complexes facilitate chromosome folding and segregation in eukaryotes, presumably by DNA...

    Michael Taschner, Stephan Gruber in Nature Structural & Molecular Biology
    Article Open access 03 April 2023
  17. Chromosome Conformation Capture for Large Genomes

    The gigantic 32Gb Axolotl genome inspires fascinating questions such as: how such a big genome is organized and packed in nuclei and how regulation...
    Akane Kawaguchi, Elly M. Tanaka in Salamanders
    Protocol 2023
  18. Targeted Sequencing in Coffee with the Daicel Arbor Biosciences Exome Capture Kit

    Exome Capture is a molecular biology technique that, in combination with Next Generation DNA sequencing technologies (NGS), allows for selectively...
    Chapter Open access 2023
  19. Comparison of capture-based mtDNA sequencing performance between MGI and illumina sequencing platforms in various sample types

    Background

    Mitochondrial genome abnormalities can lead to mitochondrial dysfunction, which in turn affects cellular biology and is closely associated...

    Zehui Feng, Fan Peng, ... Xu Guo in BMC Genomics
    Article Open access 08 January 2024
  20. Characterizing chromatin interactions of regulatory elements and nucleosome positions, using Hi-C, Micro-C, and promoter capture Micro-C

    Background

    Regulatory elements such as promoters, enhancers, and insulators interact each other to mediate molecular processes. To capture chromatin...

    Beoung Hun Lee, Zexun Wu, Suhn K. Rhie in Epigenetics & Chromatin
    Article Open access 21 December 2022
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