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Establishment of SYBR green I-based quantitative real-time polymerase chain reaction for the rapid detection of a novel Chaphamaparvovirus in cats
Feline parvovirus causes infectious diseases, and Chaphamaparvovirus is a novel type of feline parvovirus. The present study aims to establish a...
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Simultaneous detection of feline parvovirus and feline bocavirus using SYBR Green I-based duplex real-time polymerase chain reaction
Since both feline parvovirus (FPV) and feline bocavirus (FBoV) can cause diarrhea in cats, it is difficult to distinguish them clinically. This study...
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Nuclease Enrichment and qPCR Detection of Rare Nucleotide Variants
The emergence of circulating DNA analysis in blood during the past decade has responded to the need for noninvasive alternatives to classical tissue...
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Development of a SYBR Green-based RT-qPCR assay for the detection of Indian citrus ringspot virus
The Indian citrus ringspot virus (ICRSV) that causes ringspot disease, especially to ‘Kinnow mandarin’ hampers the sustainability of crop production....
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Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection
Feline bocavirus 1 (FBoV-1) may be associated with diarrhea in cats. In this study, a SYBR Green I-based quantitative polymerase chain reaction...
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Accurate Absolute Quantification of Bacterial Populations in Mixed Cultures by qPCR
Quantitative PCR (qPCR) is a well-established technique that allows to accurately quantify nucleic acids or proteins, being widely used in several...
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Interlaboratory study on real-time PCR detection and quantification of the European anglerfish, pike, and seabream parvalbumin gene
This study presents a large-scale interlaboratory comparison (ILC) aimed at detecting and quantifying DNA from two European anglerfish ( Lophius...
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TaqMan-based real-time polymerase chain reaction for the detection of feline chaphamaparvovirus
Feline chaphamaparvovirus (FeChPV) is a new viral strain detected in Chinese Mainland in recent years. The symptoms mainly include diarrhea and...
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Multiplex Real-Time PCR for the Detection of Shiga Toxin-Producing Escherichia coli in Foods
Shiga toxin-producing Escherichia coli (STECShiga Toxin-producing (STEC)) is a group of human foodborne pathogens transmitted to humans through the...
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Development of molecular markers based on real-time PCR to detect flax and sesame in commercial amaranth products
Amaranthus , Sesamum indicum , and Linum usitatissimum are the most popular oilseed grains worldwide. Protein-rich Amaranthus contains bioactive...
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Development of a TaqMan real-time PCR assay with an internal amplification control for detecting trace amounts of allergenic mango (Mangifera indica) in commercial food products
Mango ( Mangifera indica ) is a widely enjoyed tropical fruit that is rich in numerous nutrients, but it is also a common allergenic fruit that can...
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A normalized model based on Taqman real-time PCR assay for quantitative comparison of chicken adulteration in raw and heat-treated hamburgers
Mislabeling of hamburgers with undeclared chicken has frequently been reported. A fast TaqMan real-time PCR assay was developed to estimate chicken...
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Fluorescence quenching of the SYBR Green I-dsDNA complex by in situ generated magnetic ionic liquids
Magnetic ionic liquids (MILs) with metal-containing cations are promising extraction solvents that provide fast and high efficiency extraction of...
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Validation of a simplified small-scale DNA extraction protocol from wine by quantitative real-time PCR
In the present study, we compared a simplified small-scale purification protocol to obtain DNA admixtures out of wine, with our large-scale published...
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Improving sustainable hydrogen production from green waste: [FeFe]-hydrogenases quantitative gene expression RT-qPCR analysis in presence of autochthonous consortia
BackgroundBio-hydrogen production via dark fermentation of low-value waste is a potent and simple mean of recovering energy, maximising the...
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Use of RT-qPCR for Measuring the Expression of the Fecal MicroRNA from the Consumption of Pomegranate Fruit Juice
The ongoing process involves the modification and improvement of the stool test that has been validated for the purpose of diagnostic screening of...
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Assessment of Inflammation in Animal Models (Quantification of TNFA, IFNG, IL4, and IL10 mRNAs by Real-Time PCR)
Cytokines are known to play a key regulatory function in immune responses. The commencement or progression of immunopathology in different disorders...
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Rapid detection of EGFR mutation in CTCs based on a double spiral microfluidic chip and the real-time RPA method
Circulating tumor cells (CTCs) are cells shed from primary or metastatic tumors and spread into the peripheral bloodstream. Mutation detection in...
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Engineering Camelina sativa Seeds as a Green Bioreactor for the Production of Affordable Human Pro-insulin that Demonstrates Anti-diabetic Efficacy in Rats
The current production of recombinant insulin via fermenter-based platforms ( Escherichia coli and yeast) could not fulfill its fast-growing...
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Quantitative PCR (qPCR) Reveals that the Aflatoxin-Free Pistachio Samples Can Be Potentially Contaminated with Fungal Materials
Aflatoxins (AF) are the most potent group of mycotoxins that are produced by certain ascomycete fungi. These toxins can contaminate a large board of...
