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Biosafety Assessment of Probiotic Potential

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  • © 2022

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Editors:
  1. Mitesh Kumar Dwivedi

    1. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, India

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  2. Natarajan Amaresan

    1. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, India

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  3. A. Sankaranarayanan

    1. Department of Life Sciences, Sri Sathya Sai University for Human Excellence, Kalaburagi, India

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  4. Rasheedunnisa Begum

    1. Department of Biochemistry, The Maharaja Sayajirao University of Baroda, Vadodara, India

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods and Protocols in Food Science (MPFS)

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About this book

This volume provides methods on procedures for assessing the biosafety aspects of probiotics. Chapters are divided into five parts detailing in vitro biosafety assessment, biogenic amine production, D-lactic acid production, toxin production, production of various enzymes, determination of toxicity, mutagenicity, virulence genes, capsule formation, hemolytic activity, DNAse activity, bile salt deconjugation, antibiotic resistance, antibiotic resistance gene transfer, mucin degradation, platelet aggregation, and in vivo biosafety assessment of probiotics including determination of  infectivity, reproductive and developmental toxicity, and evaluation of immunological parameters in animal models.

Authoritative and cutting-edge, Biosafety Assessment of Probiotic Potential aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.  


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Table of contents (39 chapters)

  1. Front Matter

    Pages i-xvii
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  2. In Vitro Biosafety Assessment of Probiotics

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Determination of Biogenic Amine Production

      • Shilpika Pandey, Archana Chaudhari, Mitesh Kumar Dwivedi
      Pages 3-15

    3. Determination of Gelatinases, Glycosidases, and Enolase Production

      • Archana Chaudhari, Shilpika Pandey, Mitesh Kumar Dwivedi
      Pages 17-27

    4. Determination of β-Glucuronidase Production

      • Dixita Panchal, Vrutika Lad, Meonis Pithawala, Natarajan Amaresan
      Pages 29-31

    5. Determination of Nitroreductase Production

      • Dixita Panchal, Vrutika Lad, Meonis Pithawala, Natarajan Amaresan
      Pages 33-37

    6. Determination of Azoreductase Production

      • Vrutika Lad, Dixita Panchal, Meonis Pithawala, Natarajan Amaresan
      Pages 39-41

    7. Determination of Hemolytic Activity

      • Vrutika Lad, Dixita Panchal, Meonis Pithawala, Mitesh Kumar Dwivedi, Natarajan Amaresan
      Pages 43-46

    8. Determination of DNAse Activity

      • Archana Chaudhari, Ruma Raghuvanshi, Mitesh Kumar Dwivedi
      Pages 47-52

    9. Determination of Bile Salts Deconjugation

      • Hemant Borase, Satish Patil, Mitesh Kumar Dwivedi, Ramar Krishnamurthy
      Pages 53-59

    10. Determination of D-Lactic Acid Production

      • Shilpika Pandey, Archana Chaudhari, Mitesh Kumar Dwivedi
      Pages 61-70

    11. Determination of Antibiotic Resistance

      • M. Veerapagu, K. R. Jeya, Ashraf Khalifa, A. Sankaranarayanan
      Pages 71-84

    12. Determination of Antibiotic Resistance Gene Transfer

      • K. R. Jeya, Ashraf Khalifa, M. Veerapagu, A. Sankaranarayanan
      Pages 85-94

    13. Determination of Toxin Production

      • Urjita V. Sheth
      Pages 95-106

    14. Detection of Toxin Genes by PCR Based Methods

      • Urjita V. Sheth
      Pages 107-121

    15. Evaluation of Pathogenicity Potential by Phenotypic and Genotypic Methodologies

      • Teresa Semedo-Lemsaddek, Maria João Fraqueza
      Pages 123-136

    16. Determination of Toxicity Through Cytotoxicity Assays

      • Urjita V. Sheth
      Pages 137-147

    17. In Vitro Evaluation of the Nitric Oxide Pathway

      • Hemant Borase, Satish Patil, Dhruti Amin, Mitesh Kumar Dwivedi
      Pages 149-155

    18. Assessment of Capsule Formation

      • Rushabh Shah, Natarajan Amaresan, Mitesh Kumar Dwivedi
      Pages 157-163

    19. Assessment of Platelet Aggregation

      • Karthick Raja Arulprakasam, Dhanasekaran Dharumadurai
      Pages 165-170
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Editors and Affiliations

  • C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, India

    Mitesh Kumar Dwivedi, Natarajan Amaresan

  • Department of Life Sciences, Sri Sathya Sai University for Human Excellence, Kalaburagi, India

    A. Sankaranarayanan

  • Department of Biochemistry, The Maharaja Sayajirao University of Baroda, Vadodara, India

    Rasheedunnisa Begum

Bibliographic Information

  • Book Title: Biosafety Assessment of Probiotic Potential

  • Editors: Mitesh Kumar Dwivedi, Natarajan Amaresan, A. Sankaranarayanan, Rasheedunnisa Begum

  • Series Title: Methods and Protocols in Food Science

  • DOI: https://doi.org/10.1007/978-1-0716-2509-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2508-8Published: 25 August 2022

  • Softcover ISBN: 978-1-0716-2511-8Published: 25 August 2022

  • eBook ISBN: 978-1-0716-2509-5Published: 24 August 2022

  • Series ISSN: 2662-950X

  • Series E-ISSN: 2662-9518

  • Edition Number: 1

  • Number of Pages: XVII, 412

  • Number of Illustrations: 16 b/w illustrations, 19 illustrations in colour

  • Topics: Food Science

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