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Evaluation of homochiral zeolitic imidazolate framework-8 supported open-tubular column by miniaturized capillary electrochromatography with amperometric detection
A novel kind of chiral open-tubular (OT) column was established with homochiral zeolitic imidazolate framework-8 nanomaterials using L-histidine as...
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On-line Method for Montelukast Determination in Bile Salt Medium with Multivariate Optimization and Fluorescent Detection
AbstractThe present development involved a flow injection strategy using a mini-column of multiwalled carbon nanotubes and fluorescent detection (λ ex ...
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Analysis of N7-(2-carbamoyl-2-hydroxyethyl)guanine in dried blood spot after food exposure by Ultra High Performance Liquid Chromatography–Tandem Mass Spectrometry
N 7 -(2-carbamoyl-2-hydroxyethyl)guanine (N7-CAG) is a DNA adduct formed by glycidamide, which is the metabolite of acrylamide. Acrylamide can be found...
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An affordable plasmid miniprep suitable for proficient microinjection in Caenorhabditis elegans
A variety of techniques, including CRISPR-Cas9 genome editing, have been developed to produce genetically modified cell lines and animal models. In...
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Kokumi Substances in Soybean Seeds
Certain soy-based foods such as soymilk and the roasted beans improve the taste of other food products by enhancing the sensation of thickness,...
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Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins
The ability of unevolved amino acid sequences to become biological catalysts was key to the emergence of life on Earth. However, billions of years of...
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Metaproteomic Analysis of Fecal Samples from Human Subjects and Rodent Models
Fecal metaproteomics is a useful approach to measure changes in microbial and host protein abundance and to infer which members of the gut microbiota...
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Preparation of electrospun polyvinylidene fluoride/amidoximized polyacrylonitrile nanofibers for trace metal ions removal from contaminated water
In this study, a mixture of polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN), and dimethylformamide (DMF) as solvent were reacted with...
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Miniaturized Extraction Method Based on Bead Beating Tissuelyser for the Analysis of Pharmaceuticals in Seafood
Pharmaceuticals (PhACs) are considered pseudo-persistent contaminants because their removal and/or degradation surpass their continue introduction...
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Immune Cell Lineage-Specific Chimerism Testing by Next-Generation Sequencing for Engraftment Monitoring After Allogeneic Hematopoietic Stem Cell Transplantation
Chimerism is an unusual state in which a person’s body comprises cells from genetically different people. Chimerism testing allows monitoring for the...
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Cell Subset-Specific Chimerism Testing by Short Tandem Repeats Analysis for Engraftment Monitoring After Hematopoietic Stem Cell Transplantation
Chimerism is the unique state when cells from genetically different individuals coexist. Chimerism testing allows measuring the donor and recipient...
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Heme o Isolation and Analysis Using Heme o Synthase Heterologously Expressed in Escherichia coli
Heme o is an Fe–porphyrin involved in the majority of aerobic respiration pathways found in all three domains of life. In eukaryotes and most aerobic...
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A Validated Method to Prepare Stable Tau Oligomers
There is growing evidence that tau oligomers are a major pathological species in a number of tauopathies including Alzheimer’s disease. However, it...
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Assessment of Intracellular Compartmentalization of RNA Nanostructures
Nucleic acid nanoparticles (NANPs) are extensively investigated as diagnostic and therapeutic tools. These innovative particles can be composed of...
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Expression of Multispecific Antibodies
Bi- and multispecific antibody formats allow the development of new therapeutic strategies to address previously unmet medical needs. However, due to...
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In-Depth Proteomic Analysis of Blood Circulating Small Extracellular Vesicles
Circulating small extracellular vesicles (sEVs), also called exosomes, are key players in the investigation of cell–cell communication mechanisms and...
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Oxidized carbon nanotubes as sorbent for miniaturized solid-phase extraction of progestins from environmental water samples prior to their determination by HPLC-UV
A solid-phase extraction method is presented for micro-extraction of three progestins (levonorgestrel, 19-norethisterone acetate and...
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Analysis of Caenorhabditis Protein Glycosylation
Glycoproteins result from post-translational modification of proteins by glycans attached to certain side chains, with possible heterogeneity due to...
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Immobilized Artificial Membrane Chromatography Using Acetonitrile-Rich Mobile Phase for Comparison of Retention Properties Between Phospholipidosis-Inducing and Non-inducing Basic Drugs
Immobilized artificial membrane chromatography has been reported providing a rough estimation of phospholipidosis induction risk of drugs....
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Improved Coverage of the N-Terminome by Combining ChaFRADIC with Alternative Proteases
Many proteolytic cleavage events cannot be covered with conventional trypsin-based N-terminomics workflows. These typically involve the...